PMID- 15150553
OWN - NLM
STAT- MEDLINE
DCOM- 20040715
LR  - 20220309
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 90
IP  - 11
DP  - 2004 Jun 1
TI  - Absence of human T-cell lymphotropic virus type I and human foamy virus in 
      thymoma.
PG  - 2181-5
AB  - The cause of thymoma, a rare malignancy of thymic epithelial cells, is unknown. 
      Recent studies have reported the detection of DNA from human T-cell lymphotropic 
      virus type I (HTLV-I) and human foamy virus (HFV) in small numbers of thymoma 
      tumours, suggesting an aetiologic role for these retroviruses. In the present 
      study, we evaluated 21 US thymoma patients and 20 patients with other cancers for 
      evidence of infection with these viruses. We used the polymerase chain reaction 
      to attempt to amplify viral DNA from tumour tissues, using primers from the pol 
      and tax (HTLV-I) and gag and bel1 (HFV) regions. In these experiments, we did not 
      detect HTLV-I or HFV DNA sequences in any thymoma or control tissues, despite 
      adequate sensitivity of our assays (one HTLV-I copy per 25 000 cells, one HFV 
      copy per 7500 cells). Additionally, none of 14 thymoma patients evaluated 
      serologically for HTLV I/II infection was positive by enzyme-linked immunoassay 
      (ELISA), while five (36%) had indeterminate Western blot reactivity. In 
      comparison, one of 20 US blood donors was HTLV-I/II ELISA positive, and nine 
      (45%) donors, including the ELISA-positive donor, had indeterminate Western blot 
      reactivity. Western blot patterns varied across individuals and consisted mostly 
      of weak reactivity. In conclusion, we did not find evidence for the presence of 
      HTLV-I or HFV in US thymoma patients.
FAU - Li, H
AU  - Li H
AD  - Viral Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National 
      Cancer Institute, Department of Health and Human Services, 6120 Executive Blvd., 
      EPS 8010, Rockville, MD 20892, USA.
FAU - Loehrer, P J Sr
AU  - Loehrer PJ Sr
FAU - Hisada, M
AU  - Hisada M
FAU - Henley, J
AU  - Henley J
FAU - Whitby, D
AU  - Whitby D
FAU - Engels, E A
AU  - Engels EA
LA  - eng
GR  - N01CO12400/CA/NCI NIH HHS/United States
GR  - N01 CO 12400/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blotting, Western
MH  - DNA, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HTLV-I Infections/*complications
MH  - Human T-lymphotropic virus 1/genetics/isolation & purification/*pathogenicity
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Retroviridae Infections/*complications
MH  - Spumavirus/genetics/isolation & purification/*pathogenicity
MH  - Thymoma/etiology/*virology
MH  - Thymus Neoplasms/etiology/*virology
PMC - PMC2409482
EDAT- 2004/05/20 05:00
MHDA- 2004/07/16 05:00
PMCR- 2005/05/04
CRDT- 2004/05/20 05:00
PHST- 2004/05/20 05:00 [pubmed]
PHST- 2004/07/16 05:00 [medline]
PHST- 2004/05/20 05:00 [entrez]
PHST- 2005/05/04 00:00 [pmc-release]
AID - 6601841 [pii]
AID - 10.1038/sj.bjc.6601841 [doi]
PST - ppublish
SO  - Br J Cancer. 2004 Jun 1;90(11):2181-5. doi: 10.1038/sj.bjc.6601841.