PMID- 15151201
OWN - NLM
STAT- MEDLINE
DCOM- 20040603
LR  - 20220309
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 121
IP  - 5
DP  - 2004 May
TI  - Enhanced accuracy and reliability of HER-2/neu immunohistochemical scoring using 
      digital microscopy.
PG  - 620-30
AB  - We evaluated the HER-2/neu status of 129 invasive breast cancer specimens for 
      gene amplification by fluorescence in situ hybridization (FISH) and protein 
      overexpression by immunohistochemical analysis. Each immunohistochemically 
      stained slide was interpreted on a standard microscope independently by 10 
      pathologists. Separately, each pathologist reviewed the same slide set with the 
      assistance of digital microscopy. A total of 1,258 manual immunohistochemical 
      scores and 1,269 digital microscopy immunohistochemical scores were completed. 
      When the same 10 pathologists scored the same immunohistochemical slides with the 
      assistance of digital microscopy, each reviewer improved concordance with FISH, 
      and overall concordance with immunohistochemical analysis improved significantly, 
      to 93% (P < .001). The interrater kappa was used to compare interobserver 
      agreement in HER-2 immunohistochemical scoring for manual and digital microscopy 
      interpretation. Significant improvement in interobserver agreement (kappa = 0.51 
      vs 0.86; P < .001) was achieved when HER-2 immunohistochemical analysis was 
      scored with the assistance of the digital microscope. The assistance of digital 
      microscopy improves the accuracy and reliability of HER-2 immunohistochemical 
      analysis. These data suggest that documented discrepancies between HER-2 
      immunohistochemical analysis and FISH reflect predominantly errors in manual 
      immunohistochemical interpretation as opposed to immunohistochemical reagent 
      limitations.
FAU - Bloom, Kenneth
AU  - Bloom K
AD  - Department of Pathology, Rush-Presbyterian St Luke's Medical Center, Chicago, IL, 
      USA.
FAU - Harrington, Douglas
AU  - Harrington D
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*chemistry/pathology
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Gene Amplification
MH  - Genes, erbB-2
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy/instrumentation/methods
MH  - Observer Variation
MH  - Receptor, ErbB-2/*analysis
MH  - Reproducibility of Results
EDAT- 2004/05/21 05:00
MHDA- 2004/06/04 05:00
CRDT- 2004/05/21 05:00
PHST- 2004/05/21 05:00 [pubmed]
PHST- 2004/06/04 05:00 [medline]
PHST- 2004/05/21 05:00 [entrez]
AID - 10.1309/Y73U-8X72-B68T-MGH5 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2004 May;121(5):620-30. doi: 10.1309/Y73U-8X72-B68T-MGH5.