PMID- 15151761 OWN - NLM STAT- MEDLINE DCOM- 20160423 LR - 20181130 IS - 1007-8738 (Print) IS - 1007-8738 (Linking) VI - 19 IP - 2 DP - 2003 Mar TI - [Development and application of an indirect immunofluorescence assay for the detection of serum immunoglobulin G to human herpesvirus 6]. PG - 181-2 AB - AIM: To establish an indirect immunofluorescence assay(IFA) for detection of serum IgG antibody against human herpesvirus 6(HHV-6). METHODS: Cord blood mononuclear cells infected by HHV-6 strain CN(5) were used to prepare cell antigen smear, so as to establish an IFA and make an epidemical investigation on serum specimens of child-bearing age, women. RESULTS: The specificity of the IFA for HHV-6 was confirmed by absorption assay(test). The IFA detection showed that in serum specimens from 116 cases of child-bearing age, women, the positive rate of anti-HHV-6 IgG was 72.4% and geometric mean titer(GMT) was 1:61. The positive rate and GMT of serum anti-HHV-6 IgG had no differences between pregnant women and unpregnant women, neither among pregnant women at different pregnant stages. CONCLUSION: A specific IFA has been developed successfully for epidemical investigation of HHV-6 infection rate in child-bearing women. FAU - Liu, Jun-Lian AU - Liu JL AD - Department of Dermatology, 306th Hospital of PLA, Beijing 100101, China. FAU - Xu, Zhi-Kai AU - Xu ZK FAU - Wang, Hong-Ying AU - Wang HY FAU - Wang, De-Tang AU - Wang DT FAU - Zhang, Fang-Ling AU - Zhang FL FAU - Yang, Qiao-Xin AU - Yang QX FAU - Yao, Kun AU - Yao K LA - chi PT - Journal Article PL - China TA - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi JT - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JID - 101139110 RN - 0 (Antibodies, Viral) RN - 0 (Immunoglobulin G) SB - IM MH - Antibodies, Viral/blood MH - *Fluorescent Antibody Technique, Indirect MH - Herpesviridae Infections MH - *Herpesvirus 6, Human MH - Humans MH - Immunoglobulin G/blood EDAT- 2004/05/21 05:00 MHDA- 2016/04/24 06:00 CRDT- 2004/05/21 05:00 PHST- 2004/05/21 05:00 [pubmed] PHST- 2016/04/24 06:00 [medline] PHST- 2004/05/21 05:00 [entrez] PST - ppublish SO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Mar;19(2):181-2.