PMID- 15151923
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20181130
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 170
IP  - 5
DP  - 2004 Sep 1
TI  - Higher prostaglandin e2 production by dendritic cells from subjects with asthma 
      compared with normal subjects.
PG  - 485-91
AB  - Dendritic cells (DCs) are thought to play an important role in the pathogenesis 
      of allergic disorders through their ability to interact with T cells to initiate 
      and amplify helper T cell Type 2 immune responses. The mechanisms by which this 
      occurs are not completely understood, nor is it clear whether DC function differs 
      between normal individuals and individuals with asthma. We compared the function 
      of DCs from 10 subjects with allergic asthma and 10 normal individuals, focusing 
      on the production of prostaglandin E (PGE) 2, interleukin (IL)-10, and IL-12 p70, 
      mediators that play an important role in helper T cell Type 1/Type 2 
      polarization. Monocyte-derived DCs were established by culturing monocytes with 
      granulocyte-macrophage colony-stimulating factor and IL-4 for 7 days, and then 
      stimulated with LPS plus IFN-gamma. PGE2, IL-10, and IL-12 production was 
      evaluated by ELISA, whereas cyclooxygenase-1, and -2 messenger RNA expression was 
      analyzed using reverse transcription-polymerase chain reaction. LPS-stimulated 
      monocyte-derived DCs from individuals with asthma exhibited increased PGE2 and 
      IL-10 production, but equivalent IL-12 p70 synthesis, when compared with DCs from 
      normal subjects. Increased PGE2 synthesis by DCs from subjects with asthma was 
      associated with an increase in cyclooxygenase-2 messenger RNA expression. These 
      findings support the notion that DC function is significantly altered in allergic 
      asthma.
FAU - Long, Julie A
AU  - Long JA
AD  - Asthma and Allergy Research Institute, University of Western Australia, West 
      Perth, WA, Australia.
FAU - Fogel-Petrovic, Mirjana
AU  - Fogel-Petrovic M
FAU - Knight, Darryl A
AU  - Knight DA
FAU - Thompson, Philip J
AU  - Thompson PJ
FAU - Upham, John W
AU  - Upham JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040519
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protein Subunits)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Asthma/*metabolism
MH  - Case-Control Studies
MH  - Cell Culture Techniques
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Dinoprostone/*biosynthesis
MH  - Female
MH  - Humans
MH  - Interferon-gamma/physiology
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Protein Subunits/metabolism
EDAT- 2004/05/21 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/05/21 05:00
PHST- 2004/05/21 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/05/21 05:00 [entrez]
AID - 200311-1595OC [pii]
AID - 10.1164/rccm.200311-1595OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2004 Sep 1;170(5):485-91. doi: 
      10.1164/rccm.200311-1595OC. Epub 2004 May 19.