PMID- 15153640 OWN - NLM STAT- MEDLINE DCOM- 20040728 LR - 20071114 IS - 1064-3745 (Print) IS - 1064-3745 (Linking) VI - 270 DP - 2004 TI - FISH mapping for helminth genome. PG - 379-94 AB - Basic techniques for fluorescence in situ hybridization (FISH) mapping that have been used in genome projects on schistosomes and filariae are introduced. The chapter shows techniques specific for bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones and includes experiences of chromosome preparation, DNA labeling, hybridization, microscopy, and localization of BAC clones. FAU - Hirai, Hirohisa AU - Hirai H AD - Center for Human Evolution Modeling Research, Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan. FAU - Hirai, Yuriko AU - Hirai Y LA - eng GR - 1-U01-AI48828-01/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Animals MH - *Genome MH - Helminths/*genetics MH - In Situ Hybridization, Fluorescence/*methods EDAT- 2004/05/22 05:00 MHDA- 2004/07/29 05:00 CRDT- 2004/05/22 05:00 PHST- 2004/05/22 05:00 [pubmed] PHST- 2004/07/29 05:00 [medline] PHST- 2004/05/22 05:00 [entrez] AID - 1-59259-793-9:379 [pii] AID - 10.1385/1-59259-793-9:379 [doi] PST - ppublish SO - Methods Mol Biol. 2004;270:379-94. doi: 10.1385/1-59259-793-9:379.