PMID- 15153681
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20190607
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 53
IP  - 2
DP  - 2004 Apr
TI  - The susceptibility of pregnant mice to encephalomyocarditis (EMC) virus infection 
      on different days of gestation.
PG  - 165-9
AB  - Pregnant mice of the BALB/c CrSlc strain were experimentally infected with the D 
      variant of encephalomyocarditis virus (EMC-D, 5 x 10(2) PFU/head) on three 
      different gestational days (GD). Mice were intraperitoneally inoculated with 
      EMC-D on 11, 13 and 15 GD and sacrificed 3 days post inoculation. There was no 
      significant difference in the fetal mortality among all inoculation groups. 
      Placenta showed higher virus titer than fetus and dam's serum in all inoculation 
      groups, and the virus titer of the fetus was lowest in the 15GD group. 
      Histopathological changes and signals of viral RNAs detected by in situ 
      hybridization were observed almost restricted to the spongiotrophoblast layer of 
      the placenta in all inoculation groups, and the signals were strongest in the 
      11GD group. In the fetus of the11GD group, signals of viral RNAs were also seen 
      in myocardium and hepatocytes. Ultrastructurally, intracytoplasmic aggregations 
      of virus-like particles in crystalline array were observed in trophoblast cells 
      and giant cells in the spongiotrophoblast layer in all inoculation groups.
FAU - Nakayama, Yumi
AU  - Nakayama Y
AD  - Department of Veterinary Pathology, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, Japan.
FAU - Su, Weiping
AU  - Su W
FAU - Katayama, Kei-Ichi
AU  - Katayama K
FAU - Nakayama, Hiroyuki
AU  - Nakayama H
FAU - Doi, Kunio
AU  - Doi K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Cardiovirus Infections/blood/pathology/*virology
MH  - Disease Models, Animal
MH  - Disease Susceptibility/virology
MH  - Encephalomyocarditis virus/*genetics
MH  - Female
MH  - Fetus/virology
MH  - Gestational Age
MH  - Histological Techniques
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred BALB C/*virology
MH  - Placenta/ultrastructure/virology
MH  - Pregnancy
MH  - *Pregnancy, Animal
MH  - RNA, Viral/genetics
EDAT- 2004/05/22 05:00
MHDA- 2004/07/21 05:00
CRDT- 2004/05/22 05:00
PHST- 2004/05/22 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2004/05/22 05:00 [entrez]
AID - 10.1538/expanim.53.165 [doi]
PST - ppublish
SO  - Exp Anim. 2004 Apr;53(2):165-9. doi: 10.1538/expanim.53.165.