PMID- 15155648
OWN - NLM
STAT- MEDLINE
DCOM- 20040623
LR  - 20181113
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 72
IP  - 6
DP  - 2004 Jun
TI  - A novel lectin, DltA, is required for expression of a full serum resistance 
      phenotype in Haemophilus ducreyi.
PG  - 3418-28
AB  - Haemophilus ducreyi, the causative agent of chancroid, is highly resistant to the 
      complement-mediated bactericidal activity of normal human serum (NHS). 
      Previously, we identified DsrA (for ducreyi serum resistance A), a major factor 
      required for expression of the serum resistance phenotype in H. ducreyi. We 
      describe here a second outer membrane protein, DltA (for ducreyi lectin A), which 
      also contributes to serum resistance in H. ducreyi. Isogenic dltA mutants, 
      constructed in 35000HP wild-type and FX517 dsrA backgrounds, were more 
      susceptible to the bactericidal effects of NHS than each respective parent, 
      demonstrating the additive effect of the mutations. Furthermore, expression of 
      dltA in H. influenzae strain Rd rendered this highly susceptible strain partially 
      resistant to 5% NHS compared to a vector-control strain. Although primary basic 
      local alignment search tool analysis of the dltA open reading frame revealed no 
      close bacterial homologue, similarity to the beta-chain of the eukaryotic lectin 
      ricin was noted. DltA shares highly conserved structural motifs with the ricin 
      beta chain, such as cysteines and lectin-binding domains. To determine whether 
      dltA was a lectin, ligand blots and affinity chromatography experiments were 
      performed. DltA was affinity purified on immobilized lactose and 
      N-acetylgalactosamine, and N-glycosylated but not glycosidase-treated model 
      glycoproteins bound DltA. These data indicate that DltA is a lectin with 
      specificity for lactose-related carbohydrates (CHO) and is important for H. 
      ducreyi serum resistance.
FAU - Leduc, Isabelle
AU  - Leduc I
AD  - Department of Medicine, School of Medicine, University of North Carolina, Chapel 
      Hill, North Carolina 27599, USA.
FAU - Richards, Patricia
AU  - Richards P
FAU - Davis, Crystal
AU  - Davis C
FAU - Schilling, Birgit
AU  - Schilling B
FAU - Elkins, Christopher
AU  - Elkins C
LA  - eng
SI  - GENBANK/AE017143
GR  - U19 AI031496/AI/NIAID NIH HHS/United States
GR  - AI31496/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Lectins)
RN  - 0 (Peptides)
RN  - 0 (Protease Inhibitors)
RN  - 67076-74-8 (globomycin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Outer Membrane Proteins/chemistry/genetics/*metabolism
MH  - *Blood Bactericidal Activity
MH  - *Gene Expression Regulation, Bacterial
MH  - Haemophilus ducreyi/*immunology/pathogenicity
MH  - Humans
MH  - Lectins/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Peptides/pharmacology
MH  - Phenotype
MH  - Protease Inhibitors/pharmacology
PMC - PMC415671
EDAT- 2004/05/25 05:00
MHDA- 2004/06/24 05:00
PMCR- 2004/06/01
CRDT- 2004/05/25 05:00
PHST- 2004/05/25 05:00 [pubmed]
PHST- 2004/06/24 05:00 [medline]
PHST- 2004/05/25 05:00 [entrez]
PHST- 2004/06/01 00:00 [pmc-release]
AID - 72/6/3418 [pii]
AID - 1521-03 [pii]
AID - 10.1128/IAI.72.6.3418-3428.2004 [doi]
PST - ppublish
SO  - Infect Immun. 2004 Jun;72(6):3418-28. doi: 10.1128/IAI.72.6.3418-3428.2004.