PMID- 15157656
OWN - NLM
STAT- MEDLINE
DCOM- 20040819
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 560
IP  - 2
DP  - 2004 Jun 13
TI  - Clastogenic effect of ethanol in chronic and abstinent alcoholics.
PG  - 187-98
AB  - Alcoholism is one of the main causes of damage for human health, being relevant 
      to study the induction of chromosomal aberrations (CA) by ethanol, and to 
      investigate the individual susceptibility to diseases caused by alcoholism. A 
      cytogenetic study was performed in human peripheral blood lymphocytes of 29 heavy 
      chronic alcoholics, 11 alcoholics in abstinence, and 10 controls. The values of 
      the chromosomal aberrations, mitotic indexes (MI) and proliferation indexes (PI) 
      were determined. A molecular cytogenetic study was also carried out using 
      fluorescence in situ hybridization (FISH) method with DNA library probes for 
      chromosomes 1, 3 and 6, in lymphocytes from chronic alcoholic individuals in 
      comparison with a control group. The results showed that the CA frequencies for 
      chronic alcoholics (5.15 CA/100 cells) and alcoholics in abstinence (3.87 CA/100 
      cells) were higher than those obtained for control individuals (1.72 CA/100 
      cells). The mean translocation frequencies (equivalent to the genome) were 
      calculated for six chronic alcoholics (0.267 translocations/100 cells) and six 
      alcoholics in abstinence (0.167 translocations/100 cells), whose values were 
      significantly higher than those observed for six control individuals (0.067 
      translocations/100 cells). The CA frequencies were not statistically different 
      when smoker and non-smoker alcoholics were compared, indicating that although the 
      smoking habit had significantly increased (four-fold) the CA frequency in healthy 
      control individuals, a lack of interaction effect was observed within the group 
      of alcoholics when smokers and non-smokers were compared. The CA frequencies 
      presented by alcoholics in abstinence were similar to those obtained for chronic 
      alcoholics. Therefore, chronic ethanol intoxication can lead to chromosome damage 
      and disturbances in the metabolism of endogenous and exogenous compounds, which 
      may persist for a long time, and constitute a relevant factor of risk for the 
      development of neoplasias.
FAU - Burim, Regislaine Valeria
AU  - Burim RV
AD  - Department of Genetics, Faculty of Medicine of Ribeirao Preto, Sao Paulo 
      University, Ribeirao Preto, Sao Paulo, Brazil. cstaka@usp.br
FAU - Canalle, Renata
AU  - Canalle R
FAU - Takahashi, Catarina Satie
AU  - Takahashi CS
FAU - Tavares, Denise Crispim
AU  - Tavares DC
FAU - Martinelli Ad, Ana de Lourdes Candolo
AU  - Martinelli Ad Ade L
FAU - Sakamoto-Hojo, Elza Tiemi
AU  - Sakamoto-Hojo ET
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcoholism/*genetics
MH  - Case-Control Studies
MH  - Chromosome Aberrations
MH  - Chronic Disease
MH  - Ethanol/*toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mutagens/*toxicity
MH  - Translocation, Genetic
EDAT- 2004/05/26 05:00
MHDA- 2004/08/20 05:00
CRDT- 2004/05/26 05:00
PHST- 2002/06/26 00:00 [received]
PHST- 2004/02/12 00:00 [revised]
PHST- 2004/03/10 00:00 [accepted]
PHST- 2004/05/26 05:00 [pubmed]
PHST- 2004/08/20 05:00 [medline]
PHST- 2004/05/26 05:00 [entrez]
AID - S1383571804000774 [pii]
AID - 10.1016/j.mrgentox.2004.03.004 [doi]
PST - ppublish
SO  - Mutat Res. 2004 Jun 13;560(2):187-98. doi: 10.1016/j.mrgentox.2004.03.004.