PMID- 15158077
OWN - NLM
STAT- MEDLINE
DCOM- 20040823
LR  - 20231213
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 150
IP  - 2
DP  - 2004 Jun 21
TI  - Prenatal alteration and distribution of the GABA(B1) and GABA(B2) receptor 
      subunit mRNAs during rat central nervous system development.
PG  - 141-50
AB  - The prenatal developmental expression changes and distribution of the 
      gamma-aminobutyric acid (GABA)(B1) and GABA(B2) receptor subunit were 
      investigated using in situ hybridization and RNase protection assay (RPA). We 
      defined a different expression pattern of GABA(B1) subunit mRNA to that of 
      GABA(B2) subunit. GABA(B1) subunit mRNA signals were moderately expressed in the 
      cerebral cortex neuroepithelium of discrete brain regions on gestational day (GD) 
      11.5 and 12.5 and were highly expressed in the brain and spinal cord on GD 13.5 
      and 15.5. However, GABA(B2) subunit mRNAs were not detected on GD 11.5 and 12.5 
      and were first weakly detected on GD 13.5. On GD 15.5, 17.5, and 19.5, these 
      subunit mRNAs were found in the retina, hippocampus, cerebral cortex, spinal 
      cord, and cerebellum area. On GD 19.5 and 21.5, these subunits mRNA signals 
      increased in the cerebral cortex, hippocampus, thalamus, and cerebellum, but 
      decreased in the spinal cord, spinal ganglion, and midbrain, reaching similar 
      levels to that of the adult brain. On GD 21.5, these subunit mRNAs were similarly 
      expressed in almost all brain areas with a higher expression level of GABA(B1) 
      subunit mRNA than GABA(B2) subunit mRNA. Our results found that GABA(B1) and 
      GABA(B2) subunit mRNAs showed different expression patterns, with the GABA(B1) 
      subunit expressed earlier and higher. We suggest that GABA(B1) and GABA(B2) 
      subunits might have a role in the fetal brain during the gestational period for 
      pre- and post-synaptogenesis, proliferation, differentiation, and neuronal 
      maturation, and GABA(B1) subunit may be more important than GABA(B2) subunit 
      during rat prenatal development.
FAU - Li, Shupeng
AU  - Li S
AD  - Division of Life Science, College of Natural Sciences and Applied Life Science 
      (Brain Korea 21), Gyeongsang National University, Chinju 660-701, South Korea.
FAU - Park, Moon Seok
AU  - Park MS
FAU - Kim, Myeong Ok
AU  - Kim MO
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Gabrr2 protein, rat)
RN  - 0 (RNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, GABA)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Receptors, GABA-B)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Central Nervous System/embryology/*metabolism
MH  - Electrophoretic Mobility Shift Assay/methods
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - In Situ Hybridization/methods
MH  - Male
MH  - Pregnancy
MH  - RNA/isolation & purification/metabolism
MH  - RNA, Complementary/chemistry
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA
MH  - Receptors, GABA-A
MH  - Receptors, GABA-B/genetics/*metabolism
EDAT- 2004/05/26 05:00
MHDA- 2004/08/24 05:00
CRDT- 2004/05/26 05:00
PHST- 2004/03/17 00:00 [accepted]
PHST- 2004/05/26 05:00 [pubmed]
PHST- 2004/08/24 05:00 [medline]
PHST- 2004/05/26 05:00 [entrez]
AID - S0165380604000975 [pii]
AID - 10.1016/j.devbrainres.2004.03.009 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 2004 Jun 21;150(2):141-50. doi: 
      10.1016/j.devbrainres.2004.03.009.