PMID- 15158280 OWN - NLM STAT- MEDLINE DCOM- 20040920 LR - 20151119 IS - 0378-1097 (Print) IS - 0378-1097 (Linking) VI - 235 IP - 1 DP - 2004 Jun 1 TI - Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system. PG - 183-9 AB - Three 16S rRNA-targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas, Hlm474 targeting several members of the genus Halomonas, and Clw844 targeting several members of the genus Colwellia, were designed. The microbial community structure and nitrogen removal ability of nitrate-containing saline wastewater treatment systems with anaerobic packed bed and fluidized bed were monitored. Direct cell counting using fluorescence in situ hybridization (FISH) images revealed that various phylogenetic groups were evenly distributed in the anaerobic packed bed whereas members of the genus Halomonas were dominant particularly in the anaerobic fluidized bed. These results suggest that the microbial communities produced by different flow conditions correlated with denitrification ability in saline industrial wastewater treatment systems. FAU - Yoshie, Sachiko AU - Yoshie S AD - Department of Chemical Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. FAU - Noda, Naohiro AU - Noda N FAU - Tsuneda, Satoshi AU - Tsuneda S FAU - Hirata, Akira AU - Hirata A FAU - Inamori, Yuhei AU - Inamori Y LA - eng PT - Journal Article PL - England TA - FEMS Microbiol Lett JT - FEMS microbiology letters JID - 7705721 RN - 0 (Industrial Waste) RN - 0 (Oligonucleotide Probes) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Water Pollutants) RN - 451W47IQ8X (Sodium Chloride) RN - N762921K75 (Nitrogen) SB - IM MH - Bioreactors/microbiology MH - Gammaproteobacteria/classification/genetics/*isolation & purification MH - Halomonas/genetics/isolation & purification MH - In Situ Hybridization, Fluorescence MH - *Industrial Waste MH - Nitrogen/*metabolism MH - Oligonucleotide Probes/*chemistry MH - Phylogeny MH - Pseudomonas/genetics/isolation & purification MH - RNA, Ribosomal, 16S/*analysis/classification MH - Sodium Chloride MH - Water Pollutants/*metabolism EDAT- 2004/05/26 05:00 MHDA- 2004/09/21 05:00 CRDT- 2004/05/26 05:00 PHST- 2004/01/22 00:00 [received] PHST- 2004/03/08 00:00 [revised] PHST- 2004/04/17 00:00 [accepted] PHST- 2004/05/26 05:00 [pubmed] PHST- 2004/09/21 05:00 [medline] PHST- 2004/05/26 05:00 [entrez] AID - S0378109704002927 [pii] AID - 10.1016/j.femsle.2004.04.033 [doi] PST - ppublish SO - FEMS Microbiol Lett. 2004 Jun 1;235(1):183-9. doi: 10.1016/j.femsle.2004.04.033.