PMID- 15159418
OWN - NLM
STAT- MEDLINE
DCOM- 20040909
LR  - 20181113
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 165
IP  - 4
DP  - 2004 May 24
TI  - MuSK is required for anchoring acetylcholinesterase at the neuromuscular 
      junction.
PG  - 505-15
AB  - At the neuromuscular junction, acetylcholinesterase (AChE) is mainly present as 
      asymmetric forms in which tetramers of catalytic subunits are associated to a 
      specific collagen, collagen Q (ColQ). The accumulation of the enzyme in the 
      synaptic basal lamina strictly relies on ColQ. This has been shown to be mediated 
      by interaction between ColQ and perlecan, which itself binds dystroglycan. Here, 
      using transfected mutants of ColQ in a ColQ-deficient muscle cell line or COS-7 
      cells, we report that ColQ clusterizes through a more complex mechanism. This 
      process requires two heparin-binding sites contained in the collagen domain as 
      well as the COOH terminus of ColQ. Cross-linking and immunoprecipitation 
      experiments in Torpedo postsynaptic membranes together with transfection 
      experiments with muscle-specific kinase (MuSK) constructs in MuSK-deficient 
      myotubes or COS-7 cells provide the first evidence that ColQ binds MuSK. 
      Together, our data suggest that a ternary complex containing ColQ, perlecan, and 
      MuSK is required for AChE clustering and support the notion that MuSK dictates 
      AChE synaptic localization at the neuromuscular junction.
FAU - Cartaud, Annie
AU  - Cartaud A
AD  - Biologie Cellulaire des Membranes, Institut Jacques Monod, UMR 7592 Centre 
      National de la Recherche Scientifique (CNRS), Universites Paris 6 and Paris 7, 
      75251 Paris, Cedex 05, France.
FAU - Strochlic, Laure
AU  - Strochlic L
FAU - Guerra, Manuel
AU  - Guerra M
FAU - Blanchard, Benoit
AU  - Blanchard B
FAU - Lambergeon, Monique
AU  - Lambergeon M
FAU - Krejci, Eric
AU  - Krejci E
FAU - Cartaud, Jean
AU  - Cartaud J
FAU - Legay, Claire
AU  - Legay C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Muscle Proteins)
RN  - 0 (Receptors, Cholinergic)
RN  - 143972-95-6 (perlecan)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - EC 3.1.1.7 (COLQ protein, human)
SB  - IM
MH  - Acetylcholinesterase/genetics/*metabolism
MH  - Animals
MH  - Binding Sites/genetics
MH  - COS Cells
MH  - Collagen/genetics/metabolism
MH  - Heparan Sulfate Proteoglycans/metabolism
MH  - Macromolecular Substances
MH  - Mice
MH  - Mice, Knockout
MH  - Muscle Fibers, Skeletal/cytology/enzymology
MH  - Muscle Proteins/genetics/metabolism
MH  - Mutation/genetics
MH  - Neuromuscular Junction/*enzymology/ultrastructure
MH  - Protein Binding/genetics
MH  - Receptor Aggregation/genetics
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, Cholinergic/*metabolism
MH  - Synaptic Membranes/*enzymology/ultrastructure
MH  - Synaptic Transmission/genetics
MH  - Transfection
PMC - PMC2172359
EDAT- 2004/05/26 05:00
MHDA- 2004/09/10 05:00
PMCR- 2004/11/24
CRDT- 2004/05/26 05:00
PHST- 2004/05/26 05:00 [pubmed]
PHST- 2004/09/10 05:00 [medline]
PHST- 2004/05/26 05:00 [entrez]
PHST- 2004/11/24 00:00 [pmc-release]
AID - jcb.200307164 [pii]
AID - 200307164 [pii]
AID - 10.1083/jcb.200307164 [doi]
PST - ppublish
SO  - J Cell Biol. 2004 May 24;165(4):505-15. doi: 10.1083/jcb.200307164.