PMID- 1516049
OWN - NLM
STAT- MEDLINE
DCOM- 19921008
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 52
IP  - 18
DP  - 1992 Sep 15
TI  - Structural chromosome 1 aberrations in transitional cell carcinoma of the 
      bladder: interphase cytogenetics combining a centromeric, telomeric, and library 
      DNA probe.
PG  - 4929-34
AB  - Fluorescence in situ hybridization (FISH) was used to study numerical and 
      structural chromosome 1 aberrations in interphase nuclei of transitional cell 
      carcinomas (TCCs) of the urinary bladder. One of the characteristic numerical 
      aberrations, as detected previously in low-grade noninvasive TCCs, included 
      trisomy for chromosome 1 (A. H. N. Hopman et al., Cancer Res., 51: 644-651, 
      1991). We examined in more detail 22 cases with a centromeric (1q12) and a 
      telomeric associated (1p36) DNA probe and with a library DNA probe from sorted 
      human chromosome 1 in single- and double-target FISH procedures. All flow 
      cytometrically determined DNA diploid TCCs (13 cases), which showed three spots 
      for 1q12 (6 cases), had two spots for 1p36. Since the library DNA probe showed 
      three separate domains in the nuclei of these cases, the additional copy for 1q12 
      could be explained as an extra chromosome 1p-, containing the 1q12 target. In the 
      flow cytometrically determined DNA tetraploid/aneuploid tumors, the results were 
      more complex. In 6 of 9 cases, we observed an overrepresentation of 1q12 as 
      compared to 1p36, also suggesting the presence of extra copies of 1p- 
      chromosomes. The results of the present study demonstrate the utility of the FISH 
      method to assess structural chromosome aberrations in interphase nuclei of solid 
      tumors.
FAU - Poddighe, P J
AU  - Poddighe PJ
AD  - Department of Molecular Cell Biology and Genetics, University of Limburg, 
      Maastricht, The Netherlands.
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Smeets, A W
AU  - Smeets AW
FAU - Vooijs, G P
AU  - Vooijs GP
FAU - Hopman, A H
AU  - Hopman AH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Carcinoma, Transitional Cell/*genetics
MH  - *Chromosome Aberrations/*pathology
MH  - *Chromosome Disorders
MH  - *Chromosomes, Human, Pair 1
MH  - DNA Probes
MH  - DNA, Satellite
MH  - Humans
MH  - Karyotyping
MH  - Microscopy, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Ploidies
MH  - Repetitive Sequences, Nucleic Acid
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 1992/09/15 00:00
MHDA- 1992/09/15 00:01
CRDT- 1992/09/15 00:00
PHST- 1992/09/15 00:00 [pubmed]
PHST- 1992/09/15 00:01 [medline]
PHST- 1992/09/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1992 Sep 15;52(18):4929-34.