PMID- 15161184
OWN - NLM
STAT- MEDLINE
DCOM- 20040709
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 52
IP  - 11
DP  - 2004 Jun 2
TI  - Simple and rapid liquid chromatography-tandem mass spectrometry confirmatory 
      assay for determining amoxicillin and ampicillin in bovine tissues and milk.
PG  - 3286-91
AB  - A simple specific and rapid confirmatory method for determining the two 
      amphoteric penicillins, that is, amoxicillin and ampicillin, in bovine muscle, 
      liver, kidney, and milk is presented. This method is based on the matrix 
      solid-phase dispersion technique with hot water as extractant followed by liquid 
      chromatography (LC)-tandem mass spectrometry. With this instrumentation, the 
      selected reaction monitoring acquisition mode with two fragmentation reactions 
      for each analyte was adopted. After acidification and filtration of the aqueous 
      extracts, 25 microL of the tissue final extracts and 50 microL of the milk final 
      extract were injected into the LC apparatus. Absolute recovery of the two 
      analytes in any biological matrix at the 50 ppb level in tissues and the 4 ppb 
      level in milk was 74-95% with relative standard deviations (RSDs) of no larger 
      than 9%. When penicillin V was used as surrogate internal standard, relative 
      recovery of the targeted compounds present in bovine tissues and milk at, 
      respectively, 25 and 2 ppb levels ranged between 100 and 106% with RSDs of no 
      larger than 11%. When fractionation of analytes by using a short chromatographic 
      run was attempted, remarkable signal weakening for the two analytes was 
      experienced. This effect was traced to polar endogenous coextractives eluted in 
      the first part of the chromatographic run that interfered with the gas-phase ion 
      formation of the two penicillins. Slowing the chromatographic run eliminated this 
      unwelcome effect. Limits of quantification of the two analytes in bovine milk 
      were estimated to be <1 ppb, whereas amoxicillin and ampicillin could be 
      quantified in bovine tissues down to 3.1 and 0.8 ppb levels, respectively.
FAU - Bogialli, Sara
AU  - Bogialli S
AD  - Dipartimento di Chimica, Universita "La Sapienza", Piazza Aldo Moro 5, 00185 
      Roma, Italy.
FAU - Capitolino, Vittorio
AU  - Capitolino V
FAU - Curini, Roberta
AU  - Curini R
FAU - Di Corcia, Antonio
AU  - Di Corcia A
FAU - Nazzari, Manuela
AU  - Nazzari M
FAU - Sergi, Manuel
AU  - Sergi M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Anti-Bacterial Agents)
RN  - 7C782967RD (Ampicillin)
RN  - 804826J2HU (Amoxicillin)
SB  - IM
MH  - Amoxicillin/analysis
MH  - Ampicillin/analysis
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Cattle
MH  - Chromatography, Liquid/*methods
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Meat/*analysis
MH  - Milk/*chemistry
MH  - Muscles/chemistry
MH  - Quality Control
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2004/05/27 05:00
MHDA- 2004/07/10 05:00
CRDT- 2004/05/27 05:00
PHST- 2004/05/27 05:00 [pubmed]
PHST- 2004/07/10 05:00 [medline]
PHST- 2004/05/27 05:00 [entrez]
AID - 10.1021/jf0499572 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2004 Jun 2;52(11):3286-91. doi: 10.1021/jf0499572.