PMID- 15162053
OWN - NLM
STAT- MEDLINE
DCOM- 20041101
LR  - 20131121
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 104
IP  - 1-4
DP  - 2004
TI  - Region-specific chromatin decondensation and micronucleus formation induced by 
      5-azacytidine in human TIG-7 cells.
PG  - 289-94
AB  - A human diploid lung fibroblast cell strain, TIG-7, has a heteromorphic 
      chromosome 15 with an extra short arm carrying a homogeneously staining region 
      (15p+hsr). We demonstrated previously that the 15p+hsr consists of an inactive 
      and G+C-rich rDNA cluster characterized by fluorescence in situ hybridization 
      (FISH) and various chromosome banding techniques. Thus, it was suggested that the 
      region could contain highly methylated DNA. To observe methylation status on the 
      target region directly under the microscope, we used a demethylating agent, 
      5-azacytidine (5-azaC), to induce decondensation of the chromatin containing 
      methylated DNA. At 24 h after treatment with 0.5 microM 5-azaC, marked 
      decondensation of the 15p+hsr was observed in almost all of the metaphases. 
      Furthermore, we observed micronuclei, which were equivalent to the rDNA of the 
      15p+hsr demonstrated by FISH in the same preparation. In contrast, the DNA 
      cross-linking agent mitomycin C (MMC) preferentially induced 15p+hsr-negative 
      micronuclei. These findings indicated that chromatin decondensation and 
      subsequent DNA strand breakage induced by the demethylating effect of 5-azaC led 
      specifically to 15p+hsr-positive micronuclei.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Satoh, T
AU  - Satoh T
AD  - Genome Medical Business Division, Olympus Corporation, Hachioji, Japan. 
      ta_sato@ot.olympus.co.jp
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Miura, K F
AU  - Miura KF
FAU - Sofuni, T
AU  - Sofuni T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Chromatin)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Satellite)
RN  - 0 (Heterochromatin)
RN  - 50SG953SK6 (Mitomycin)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Azacitidine/*pharmacology/toxicity
MH  - Cells, Cultured/drug effects/ultrastructure
MH  - Chromatin/*drug effects/ultrastructure
MH  - Chromosome Breakage
MH  - Chromosomes, Human, Pair 15/*drug effects/genetics/ultrastructure
MH  - Cross-Linking Reagents/pharmacology/toxicity
MH  - DNA Damage
MH  - DNA Methylation/drug effects
MH  - DNA, Ribosomal/drug effects
MH  - DNA, Satellite/drug effects/ultrastructure
MH  - Fibroblasts/drug effects/ultrastructure
MH  - Heterochromatin/drug effects/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung/cytology
MH  - Male
MH  - Micronucleus Tests
MH  - Mitomycin/pharmacology/toxicity
EDAT- 2004/05/27 05:00
MHDA- 2004/11/02 09:00
CRDT- 2004/05/27 05:00
PHST- 2003/09/15 00:00 [received]
PHST- 2003/12/16 00:00 [accepted]
PHST- 2004/05/27 05:00 [pubmed]
PHST- 2004/11/02 09:00 [medline]
PHST- 2004/05/27 05:00 [entrez]
AID - 77504 [pii]
AID - 10.1159/000077504 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2004;104(1-4):289-94. doi: 10.1159/000077504.