PMID- 15164987
OWN - NLM
STAT- MEDLINE
DCOM- 20040628
LR  - 20190911
IS  - 0167-594X (Print)
IS  - 0167-594X (Linking)
VI  - 67
IP  - 3
DP  - 2004 May
TI  - Interstitial infusion of carmustine in the rat brain stem with systemic 
      administration of O6-benzylguanine.
PG  - 319-26
AB  - Interstitial infusion of carmustine (BCNU) into the rat brain stem in conjunction 
      with systemic administration of O6-benzylguanine (O6-BG) was performed in an 
      effort to assess clinical tolerance. A total of 12 rats underwent stereotactic 
      cannula placement into the pontine segment of the brain stem. Six of the rats 
      underwent a 24-h infusion of BCNU (Volume of infusion [Vi] 200 microl) at its 
      maximal concentrated dose (3.3 mg/ml) in 5% dextrose water. Six additional rats 
      underwent 24-h infusion of BCNU preceded by an intraperitoneal injection of O6-BG 
      at a dose of 50 mg/kg. Serial neurological examinations were performed on all 
      animals. Histologic analyses were performed immediately or 2 weeks following 
      sacrifice. Postoperatively, there were no neurological changes in any of the 
      animals. Postmortem histological examination of the brains showed small pontine 
      cavitary lesions (ranging from 20 to 250 microm) containing variable numbers of 
      macrophages or neutrophils consistent with an inflammatory response. No changes 
      beyond these findings indicated any histological evidence of injury. These 
      finding were limited only to the cannula site and no changes beyond region of the 
      cannula tract were found. These findings indicate that interstitial infusion of 
      BCNU into the brain stem in conjunction with systemic administration of O6-BG is 
      safe in a small animal and may serve as a potential investigative strategy for 
      children with diffuse pontine gliomas.
FAU - Souweidane, Mark M
AU  - Souweidane MM
AD  - Department of Neurological Surgery, The Weill Medical College of Cornell 
      University, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. 
      mmsouwei@med.cornell.edu
FAU - Occhiogrosso, Giuseppe
AU  - Occhiogrosso G
FAU - Mark, Erika B
AU  - Mark EB
FAU - Edgar, Mark A
AU  - Edgar MA
FAU - Dunkel, Ira J
AU  - Dunkel IJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
RN  - 01KC87F8FE (O(6)-benzylguanine)
RN  - 5Z93L87A1R (Guanine)
RN  - U68WG3173Y (Carmustine)
SB  - IM
MH  - Animals
MH  - Antineoplastic Combined Chemotherapy Protocols/*administration & dosage
MH  - Brain Stem/*drug effects/metabolism/pathology
MH  - Carmustine/administration & dosage
MH  - Guanine/administration & dosage/*analogs & derivatives
MH  - Infusion Pumps
MH  - Injections, Intraperitoneal
MH  - Macrophages/drug effects/metabolism
MH  - Male
MH  - Neutrophils/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stereotaxic Techniques
EDAT- 2004/05/29 05:00
MHDA- 2004/06/29 05:00
CRDT- 2004/05/29 05:00
PHST- 2004/05/29 05:00 [pubmed]
PHST- 2004/06/29 05:00 [medline]
PHST- 2004/05/29 05:00 [entrez]
AID - 10.1023/b:neon.0000024242.59770.7a [doi]
PST - ppublish
SO  - J Neurooncol. 2004 May;67(3):319-26. doi: 10.1023/b:neon.0000024242.59770.7a.