PMID- 1516624
OWN - NLM
STAT- MEDLINE
DCOM- 19921007
LR  - 20131121
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 22
IP  - 9
DP  - 1992 Sep
TI  - HLA class I and II molecules present influenza virus antigens with different 
      kinetics.
PG  - 2339-45
AB  - Human leukocyte antigen (HLA) class I and class II molecules differ with respect 
      to their intracellular pathways and the compartments where they associate with 
      processed antigen. To study possible consequences of these differences for the 
      kinetics of antigen presentation by HLA class I and class II molecules, we 
      analyzed changes in the concentrations of free intracellular calcium ions in 
      influenza virus-specific T cell clones after recognition of specific antigen/HLA 
      complexes. HLA class II-restricted viral antigen presentation by Epstein-Barr 
      virus-transformed B lymphoblastoid cell lines (B-LCL) to CD4+ T cell clones 
      started within 1 h and showed little variability, irrespective of antigen 
      specificity or restriction element tested. In contrast, kinetics of viral antigen 
      presentation by HLA class I molecules to CD8+ T cell clones were slower and 
      differed for three antigen/HLA class I complexes tested. While B-LCL presented 
      antigen by HLA-A2 and by HLA-B37 after at least 2 h, they only started to present 
      antigen in the context of HLA-B7 after more than 4 h. This difference in kinetics 
      did not correlate with differences in bulk transport rates of HLA-A2, HLA-B37, 
      and HLA-B7, but seemed greatly influenced by differential rates of peptide 
      generation. Brefeldin A treatment of B-LCL showed for both HLA class I and class 
      II that de novo synthesized HLA molecules were involved in antigen presentation. 
      Thus, differences between intracellular pathways of HLA class I and class II 
      molecules may result in different kinetics of antigen presentation.
FAU - Kuijpers, K C
AU  - Kuijpers KC
AD  - Department of Clinical Viro-Immunology, University of Amsterdam, The Netherlands.
FAU - van Kemenade, F J
AU  - van Kemenade FJ
FAU - Hooibrink, B
AU  - Hooibrink B
FAU - Neefjes, J J
AU  - Neefjes JJ
FAU - Lucas, C J
AU  - Lucas CJ
FAU - van Lier, R A
AU  - van Lier RA
FAU - Miedema, F
AU  - Miedema F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, Viral)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antigens, Viral/*immunology
MH  - Calcium/metabolism
MH  - Clone Cells
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - Kinetics
MH  - Orthomyxoviridae/*immunology
MH  - T-Lymphocytes/immunology/metabolism
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1002/eji.1830220924 [doi]
PST - ppublish
SO  - Eur J Immunol. 1992 Sep;22(9):2339-45. doi: 10.1002/eji.1830220924.