PMID- 15166436
OWN - NLM
STAT- MEDLINE
DCOM- 20040624
LR  - 20200303
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 85
IP  - Pt 6
DP  - 2004 Jun
TI  - Identification of novel hepatitis C virus-specific cytotoxic T lymphocyte 
      epitopes by ELISpot assay using peptides with human leukocyte 
      antigen-A*2402-binding motifs.
PG  - 1521-1531
LID - 10.1099/vir.0.79801-0 [doi]
AB  - The human leukocyte antigen (HLA)-A*2402 is common in Asians. The authors 
      attempted to identify epitopes for HLA-A*2402-restricted, hepatitis C virus 
      (HCV)-specific CD8(+) T cells by an enzyme-linked immunospot (ELISpot) assay 
      using peripheral blood CD8(+) T cells from HLA-A*2402-positive hepatitis C 
      patients and synthetic HCV peptides based on HLA-A*2402-binding motifs and the 
      amino acid sequence of type 1b HCV. Ten novel epitopes were identified in five of 
      seven HLA-A*2402-positive patients with acute or short-term chronic HCV infection 
      (<3 years), but in none of four with longer-term chronic infection (>10 years). 
      Only one of the ten epitopes proved to be definitely HLA-A*2402-restricted. 
      Another epitope was identified in one of two HLA-A*2402-negative acute hepatitis 
      C patients. In two of the six patients with positive CD8(+) T cell responses, the 
      targeted epitopes were multiple. The same epitope was targeted in two patients. 
      When patients with unresolved acute HCV infection were treated with alpha 
      interferon, peripheral blood HCV-specific CD8(+) T cells decreased with 
      resolution of the hepatitis. In conclusion, CD8(+) T cell responses to HCV 
      infection are heterogeneous. One definite HLA-A*2402-restricted and ten probably 
      non-HLA-A*2402-restricted epitopes were identified. Patients with short-term HCV 
      infection are suitable for searching for novel HCV epitopes, but peripheral blood 
      HCV-specific CD8(+) T cells decrease markedly after loss of antigenic 
      stimulation.
FAU - Hakamada, Taku
AU  - Hakamada T
AD  - Division of Gastroenterology, Institute of Clinical Medicine, University of 
      Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan.
FAU - Funatsuki, Kiyomi
AU  - Funatsuki K
AD  - Research & Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories 
      Inc., 3-30-1 Shimura, Itabashi-ku, Tokyo 174-8555, Japan.
FAU - Morita, Hiroki
AU  - Morita H
AD  - Division of Gastroenterology, Omiya Medical Center, Jichi Medical School, 1-847 
      Amanuma-cho, Omiya-ku, Saitama 330-8503, Japan.
FAU - Ugajin, Takuhiro
AU  - Ugajin T
AD  - Division of Gastroenterology, Omiya Medical Center, Jichi Medical School, 1-847 
      Amanuma-cho, Omiya-ku, Saitama 330-8503, Japan.
FAU - Nakamura, Ikuo
AU  - Nakamura I
AD  - Division of Gastroenterology, Omiya Medical Center, Jichi Medical School, 1-847 
      Amanuma-cho, Omiya-ku, Saitama 330-8503, Japan.
FAU - Ishiko, Hiroaki
AU  - Ishiko H
AD  - Research & Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories 
      Inc., 3-30-1 Shimura, Itabashi-ku, Tokyo 174-8555, Japan.
FAU - Matsuzaki, Yasushi
AU  - Matsuzaki Y
AD  - Division of Gastroenterology, Institute of Clinical Medicine, University of 
      Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan.
FAU - Tanaka, Naomi
AU  - Tanaka N
AD  - Division of Gastroenterology, Institute of Clinical Medicine, University of 
      Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan.
FAU - Imawari, Michio
AU  - Imawari M
AD  - Second Department of Internal Medicine, Showa University School of Medicine, 
      1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Interferon-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Binding Sites
MH  - *Epitopes, T-Lymphocyte
MH  - HLA-A Antigens/*metabolism
MH  - Hepacivirus/*immunology
MH  - Hepatitis C/drug therapy/immunology
MH  - Humans
MH  - Interferon-alpha/therapeutic use
MH  - Interferon-gamma/biosynthesis
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2004/05/29 05:00
MHDA- 2004/06/25 05:00
CRDT- 2004/05/29 05:00
PHST- 2004/05/29 05:00 [pubmed]
PHST- 2004/06/25 05:00 [medline]
PHST- 2004/05/29 05:00 [entrez]
AID - 10.1099/vir.0.79801-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2004 Jun;85(Pt 6):1521-1531. doi: 10.1099/vir.0.79801-0.