PMID- 15169636
OWN - NLM
STAT- MEDLINE
DCOM- 20040810
LR  - 20081121
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 25
IP  - 6
DP  - 2004 Jun
TI  - Induction of systemic lupus erythematosus syndrome in BALB/c mice by immunization 
      with active chromatin.
PG  - 807-11
AB  - AIM: To establish an animal model for systemic lupus erythematosus (SLE)-like 
      syndrome in mice. METHODS: BALB/c mice were immunized with active chromatin 
      isolated from ConA-activated syngeneic spleno-lymphocytes. Plasma samples of mice 
      were tested by enzyme-linked immunosorbent assays (ELISA) for the presence of IgG 
      anti-dsDNA, -ssDNA, and anti-histone antibodies. Tumor necrosis factor-alpha 
      (TNF-alpha) in serum was measured by ELISA. Spleno-lymphocyte proliferation 
      assays and the levels of interferon-gamma (IFN-gamma) in supernatants were tested 
      respectively. Proteinuria was measured. Kidneys were examined by direct 
      immunohistochemical method and light microscopy. RESULTS: Anti-ds DNA, ssDNA, and 
      histone antibodies were induced in active chromatin-immunized mice, the 
      proliferation response of splenocytes to ConA and LPS were reduced, levels of 
      interferon-gamma in supernatants and TNF-alpha in serum were lowered. Lupus 
      nephritis was assessed by the presence of Ig deposits, glomerular pathology and 
      proteinuria. CONCLUSION: The active chromatin-induced SLE-like mouse model was 
      similar to idiopathic SLE in human.
FAU - Li, Hong
AU  - Li H
AD  - Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 
      200032, China.
FAU - Zhang, Yun-Yi
AU  - Zhang YY
FAU - Sun, Ya-Nan
AU  - Sun YN
FAU - Huang, Xi-Yi
AU  - Huang XY
FAU - Jia, Yong-Feng
AU  - Jia YF
FAU - Li, Duan
AU  - Li D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Autoantibodies)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Histones)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Autoantibodies/analysis
MH  - Cell Division
MH  - Chromatin
MH  - DNA/immunology
MH  - DNA, Single-Stranded/immunology
MH  - *Disease Models, Animal
MH  - Female
MH  - Histones/immunology
MH  - Immunoglobulin G/analysis
MH  - Interferon-gamma/blood
MH  - Kidney/pathology
MH  - Lupus Erythematosus, Systemic/*chemically induced/metabolism/pathology
MH  - Lupus Nephritis/chemically induced/pathology
MH  - Lymphocytes/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2004/06/01 05:00
MHDA- 2004/08/11 05:00
CRDT- 2004/06/01 05:00
PHST- 2004/06/01 05:00 [pubmed]
PHST- 2004/08/11 05:00 [medline]
PHST- 2004/06/01 05:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2004 Jun;25(6):807-11.