PMID- 15170848
OWN - NLM
STAT- MEDLINE
DCOM- 20040914
LR  - 20191003
IS  - 0740-3194 (Print)
IS  - 0740-3194 (Linking)
VI  - 51
IP  - 6
DP  - 2004 Jun
TI  - In vitro release of vascular endothelial growth factor from gadolinium-doped 
      biodegradable microspheres.
PG  - 1265-71
AB  - A drug delivery vehicle was constructed that could be visualized noninvasively 
      with MRI. The biodegradable polymer poly(DL-lactic-co-glycolic acid) (PLGA) was 
      used to fabricate microspheres containing vascular endothelial growth factor 
      (VEGF) and the MRI contrast agent gadolinium diethylenetriamine pentaacetic acid 
      (Gd-DTPA). The microspheres were characterized in terms of size, drug and 
      contrast agent encapsulation, and degradation rate. The PLGA microspheres had a 
      mean diameter of 48 +/- 18 microm. The gadolinium loading was 17 +/- 3 microg/mg 
      polymer and the VEGF loading was 163 +/- 22 ng/mg polymer. Electron microscopy 
      revealed that the Gd was dispersed throughout the microspheres and it was 
      confirmed that the Gd loading was sufficient to visualize the microspheres under 
      MRI. VEGF and Gd-DTPA were released from the microspheres in vitro over a period 
      of approximately 6 weeks in three phases: a burst, followed by a slow 
      steady-state, then a rapid steady-state. Biodegradable Gd-doped microspheres can 
      be effectively used to deliver drugs in a sustained manner, while being monitored 
      noninvasively with MRI.
FAU - Faranesh, Anthony Z
AU  - Faranesh AZ
AD  - Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, 
      Maryland, USA. faranesh@nih.gov
FAU - Nastley, Monet T
AU  - Nastley MT
FAU - Perez de la Cruz, Cristina
AU  - Perez de la Cruz C
FAU - Haller, Michael F
AU  - Haller MF
FAU - Laquerriere, Patrice
AU  - Laquerriere P
FAU - Leong, Kam W
AU  - Leong KW
FAU - McVeigh, Elliot R
AU  - McVeigh ER
LA  - eng
GR  - Z01 HL004608-08/Intramural NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Magn Reson Med
JT  - Magnetic resonance in medicine
JID - 8505245
RN  - 0 (Contrast Media)
RN  - 0 (Drug Carriers)
RN  - 0 (Polymers)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - K2I13DR72L (Gadolinium DTPA)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Contrast Media
MH  - *Drug Carriers
MH  - Gadolinium DTPA
MH  - Humans
MH  - In Vitro Techniques
MH  - *Infusion Pumps, Implantable
MH  - *Lactic Acid
MH  - *Microspheres
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Vascular Endothelial Growth Factor A/*pharmacokinetics/pharmacology
PMC - PMC2396272
MID - NIHMS27040
EDAT- 2004/06/02 05:00
MHDA- 2004/09/15 05:00
PMCR- 2008/05/26
CRDT- 2004/06/02 05:00
PHST- 2004/06/02 05:00 [pubmed]
PHST- 2004/09/15 05:00 [medline]
PHST- 2004/06/02 05:00 [entrez]
PHST- 2008/05/26 00:00 [pmc-release]
AID - 10.1002/mrm.20092 [doi]
PST - ppublish
SO  - Magn Reson Med. 2004 Jun;51(6):1265-71. doi: 10.1002/mrm.20092.