PMID- 15172751
OWN - NLM
STAT- MEDLINE
DCOM- 20040713
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 151
IP  - 2
DP  - 2004 Jun
TI  - Is fluorescence in situ hybridization a useful method in diagnosis of 
      polycythemia vera patients?
PG  - 139-45
AB  - Polycythemia vera (PV) is a clonal stem cell disease with trilineage myeloid 
      involvement, characterized by a growth factor-independent erythroid 
      proliferation. At the time of diagnosis, the percentage of cytogenetic 
      abnormalities using conventional cytogenetic techniques is less than 20% of all 
      PV patients. In the present study, we compare the results between conventional 
      cytogenetic methods and fluorescence in situ hybridization (FISH) probes in 31 
      untreated PV patients. The karyotypes of all 31 cases were obtained from 24-hour 
      bone marrow cell cultures. The fixed material, proceeding from conventional 
      cytogenetics cultures, was analyzed with FISH, using centromeric probes for 
      chromosomes 8 and 9 and locus-specific probes for 13q14 and 20q12 regions. Five 
      cases (17.8%) showed an abnormal karyotype with conventional cytogenetics. When 
      FISH probes were used, three alterations not detected with conventional 
      cytogenetics, were found: two cases with D20S108 deletions and one with a D13S319 
      deletion, increasing the percentage of abnormal karyotypes to 19.3%. We conclude 
      that, probably, the application of FISH with the mentioned probes, is not very 
      useful to detect cytogenetic aberrations in untreated PV patients.
FAU - Zamora, Lurdes
AU  - Zamora L
AD  - Laboratori de Citogenetica i Biologia Molecular, Servei de Patologia, Hospital 
      del Mar, Passeig Maritim 25-29, Barcelona 08003, Spain. lurdes.zamora@wanadoo.es
FAU - Espinet, Blanca
AU  - Espinet B
FAU - Florensa, Lourdes
AU  - Florensa L
FAU - Besses, Carles
AU  - Besses C
FAU - Woessner, Soledad
AU  - Woessner S
FAU - Serrano, Sergi
AU  - Serrano S
FAU - Sole, Francesc
AU  - Sole F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Polycythemia Vera/*diagnosis/genetics
EDAT- 2004/06/03 05:00
MHDA- 2004/07/14 05:00
CRDT- 2004/06/03 05:00
PHST- 2003/07/03 00:00 [received]
PHST- 2003/08/26 00:00 [revised]
PHST- 2003/09/08 00:00 [accepted]
PHST- 2004/06/03 05:00 [pubmed]
PHST- 2004/07/14 05:00 [medline]
PHST- 2004/06/03 05:00 [entrez]
AID - S0165460803003972 [pii]
AID - 10.1016/j.cancergencyto.2003.09.011 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Jun;151(2):139-45. doi: 
      10.1016/j.cancergencyto.2003.09.011.