PMID- 15172756
OWN - NLM
STAT- MEDLINE
DCOM- 20040713
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 151
IP  - 2
DP  - 2004 Jun
TI  - Comprehensive conventional and molecular cytogenetic characterization of B-CPAP, 
      a human papillary thyroid carcinoma-derived cell line.
PG  - 171-7
AB  - Cell lines derived from different thyroid tumor histotypes are useful for the in 
      vitro study of both the phenotypic and genetic features of these cancers. 
      Although karyotypic changes are known to be associated with thyroid lesions, the 
      chromosome patterns of only a few cell lines have been published. Herein, we 
      report an extensive conventional and molecular cytogenetic investigation of the 
      human papillary thyroid carcinoma derived cell line B-CPAP. Morphological studies 
      and expression of tumor markers in this cell line have been reported previously, 
      but no detailed characterization on the origin of the chromosome markers is 
      available. B-CPAP cells have a rather stable hypertriploid karyotype, with 
      chromosome polysomies and structural chromosome abnormalities featuring whole 
      chromosome arm imbalances. Chromosome banding revealed a main clone with nine 
      chromosome markers, and fluorescence in situ hybridization (FISH) with whole 
      chromosome paint (wcp), partial chromosome paint (pcp), and centromeric probes 
      clarified their origin. The use of centromeric probes provided accurate 
      refinement of the rearrangements classified as whole-arm translocations by 
      banding and FISH with wcp probes. Both chromosomal and array-based comparative 
      genomic hybridization experiments confirmed the cytogenetic characterization of 
      this cell line. Moreover, the use of fluorescence immunophenotyping and 
      interphase cytogenetics as a tool for the investigation of neoplasms (FICTION) 
      technique, which simultaneously shows nuclear ploidy and cytoplasmic 
      immunofluorescence, detailed the oncocytic feature of the cells. Intriguingly, 
      despite their origin, they lack most of the features expressed in papillary 
      thyroid tumor cells and have a chromosomal pattern reminiscent of that of a 
      subgroup of oncocytic malignant thyroid tumors.
FAU - Dettori, T
AU  - Dettori T
AD  - Dipartimento di Scienze e Tecnologie Biomediche, Universita di Cagliari, 
      Cittadella Universitaria, Monserrato, Cagliari 09042, Italy.
FAU - Frau, D V
AU  - Frau DV
FAU - Garcia, J L
AU  - Garcia JL
FAU - Pierantoni, G
AU  - Pierantoni G
FAU - Lee, C
AU  - Lee C
FAU - Hernandez, J M
AU  - Hernandez JM
FAU - Fusco, A
AU  - Fusco A
FAU - Morton, C C
AU  - Morton CC
FAU - Vanni, R
AU  - Vanni R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
EIN - Cancer Genet Cytogenet. 2004 Nov;155(1):93-4
MH  - Carcinoma, Papillary/*genetics
MH  - Cell Line, Tumor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Thyroid Neoplasms/*genetics
EDAT- 2004/06/03 05:00
MHDA- 2004/07/14 05:00
CRDT- 2004/06/03 05:00
PHST- 2003/05/28 00:00 [received]
PHST- 2003/09/05 00:00 [revised]
PHST- 2003/09/24 00:00 [accepted]
PHST- 2004/06/03 05:00 [pubmed]
PHST- 2004/07/14 05:00 [medline]
PHST- 2004/06/03 05:00 [entrez]
AID - S0165460803004370 [pii]
AID - 10.1016/j.cancergencyto.2003.09.023 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Jun;151(2):171-7. doi: 
      10.1016/j.cancergencyto.2003.09.023.