PMID- 15175640
OWN - NLM
STAT- MEDLINE
DCOM- 20040920
LR  - 20121115
IS  - 0969-7128 (Print)
IS  - 0969-7128 (Linking)
VI  - 11
IP  - 16
DP  - 2004 Aug
TI  - Antimonocyte chemoattractant protein-1 gene therapy reduces experimental in-stent 
      restenosis in hypercholesterolemic rabbits and monkeys.
PG  - 1273-82
AB  - In-stent restenosis results exclusively from neointimal hyperplasia due to 
      mechanical injury and a foreign body response to the prosthesis. Inflammation 
      mediated by monocyte chemoattractant protein-1 (MCP-1) might therefore underlie 
      in-stent restenosis. We recently devised a new strategy for anti-MCP-1 gene 
      therapy by transfecting an N-terminal deletion mutant of the MCP-1 gene into 
      skeletal muscles. We used this strategy to investigate the role of MCP-1 in 
      experimental in-stent restenosis in hypercholesterolemic rabbits and monkeys. 
      Transfection of the mutant MCP-1 gene suppressed monocyte infiltration/activation 
      in the stented arterial wall and markedly reduced the development of neointimal 
      hyperplasia. This strategy also suppressed local expression of MCP-1 and 
      inflammatory cytokines. Therefore, inhibition of MCP-1-mediated inflammation is 
      effective in reducing experimental in-stent restenosis. This strategy might be a 
      useful form of gene therapy against human in-stent restenosis.
FAU - Ohtani, K
AU  - Ohtani K
AD  - Department of Cardiovascular Medicine, Graduate School of Medical Sciences, 
      Kyushu University, Fukuoka, Japan.
FAU - Usui, M
AU  - Usui M
FAU - Nakano, K
AU  - Nakano K
FAU - Kohjimoto, Y
AU  - Kohjimoto Y
FAU - Kitajima, S
AU  - Kitajima S
FAU - Hirouchi, Y
AU  - Hirouchi Y
FAU - Li, X-H
AU  - Li XH
FAU - Kitamoto, S
AU  - Kitamoto S
FAU - Takeshita, A
AU  - Takeshita A
FAU - Egashira, K
AU  - Egashira K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gene Ther
JT  - Gene therapy
JID - 9421525
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Chemokine CCL2/*genetics
MH  - Chemokines/genetics
MH  - Chemotactic Factors/*genetics
MH  - Coronary Restenosis/prevention & control
MH  - Cytokines/genetics
MH  - Gene Expression/genetics
MH  - Genetic Therapy/*methods
MH  - Hyperplasia
MH  - Macaca fascicularis
MH  - Male
MH  - Muscle, Skeletal
MH  - Polymerase Chain Reaction/methods
MH  - Prosthesis Failure
MH  - Rabbits
MH  - Recombination, Genetic
MH  - *Stents
MH  - Transfection
EDAT- 2004/06/04 05:00
MHDA- 2004/09/21 05:00
CRDT- 2004/06/04 05:00
PHST- 2004/06/04 05:00 [pubmed]
PHST- 2004/09/21 05:00 [medline]
PHST- 2004/06/04 05:00 [entrez]
AID - 3302288 [pii]
AID - 10.1038/sj.gt.3302288 [doi]
PST - ppublish
SO  - Gene Ther. 2004 Aug;11(16):1273-82. doi: 10.1038/sj.gt.3302288.