PMID- 15178084
OWN - NLM
STAT- MEDLINE
DCOM- 20040830
LR  - 20161124
IS  - 0141-1136 (Print)
IS  - 0141-1136 (Linking)
VI  - 58
IP  - 2-5
DP  - 2004 Aug-Dec
TI  - A comparison of pollutant biomarker responses with transcriptional responses in 
      European flounders (Platicthys flesus) subjected to estuarine pollution.
PG  - 571-5
AB  - European flounders from two UK estuaries with different contaminant profiles were 
      sampled and analysed for impacts of PAHs, heavy metals and estrogenic compounds 
      by currently adopted measurement procedures used in statutory monitoring 
      programs. These showed that fish from the polluted Tyne estuary had responded to 
      all three classes of pollutants with elevations in hepatic EROD, MT and plasma 
      VTG and that there was considerable interanimal variation in the magnitude of the 
      response. Analysis of PAH metabolites in bile showed recent uptake of low 
      molecular weight PAHs. The pollutant response was also apparent when hepatic 
      levels of cytochrome P450 1A (CYP1A), metallothionein (MT) and vitellogenin (VTG) 
      mRNAs were quantified by real time PCR. Whilst there did not appear to be a 
      direct correlation between levels of gene transcripts and their protein products 
      on an individual basis, these results demonstrate that measurements of mRNA 
      levels of specific genes, or their protein products both demonstrate induction in 
      polluted environments and are thus valid measures in biomonitoring studies. 
      Procedurally, the use of the single generic technology of Q-PCR was much simpler 
      and is worth pursuing in future studies.
FAU - George, Stephen
AU  - George S
AD  - Institute of Aquaculture, University of Stirling, Stirling, Scotland FK9 4LA, UK. 
      S.G.George@stir.ac.uk
FAU - Gubbins, M
AU  - Gubbins M
FAU - MacIntosh, A
AU  - MacIntosh A
FAU - Reynolds, W
AU  - Reynolds W
FAU - Sabine, V
AU  - Sabine V
FAU - Scott, A
AU  - Scott A
FAU - Thain, J
AU  - Thain J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mar Environ Res
JT  - Marine environmental research
JID - 9882895
RN  - 0 (Biomarkers)
RN  - 0 (DNA Primers)
RN  - 0 (Estrogens)
RN  - 0 (Metals, Heavy)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vitellogenins)
RN  - 9038-94-2 (Metallothionein)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Bile/metabolism
MH  - Biomarkers
MH  - Cytochrome P-450 CYP1A1/biosynthesis
MH  - DNA Primers
MH  - Environmental Exposure
MH  - *Environmental Monitoring
MH  - Estrogens/analysis/*toxicity
MH  - Flounder/genetics/*metabolism
MH  - Gene Expression/*drug effects
MH  - Metallothionein/biosynthesis
MH  - Metals, Heavy/analysis/*toxicity
MH  - Polycyclic Aromatic Hydrocarbons/analysis/*toxicity
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Seawater
MH  - United Kingdom
MH  - Vitellogenins/biosynthesis/blood
EDAT- 2004/06/05 05:00
MHDA- 2004/08/31 05:00
CRDT- 2004/06/05 05:00
PHST- 2004/06/05 05:00 [pubmed]
PHST- 2004/08/31 05:00 [medline]
PHST- 2004/06/05 05:00 [entrez]
AID - S0141-1136(04)00098-4 [pii]
AID - 10.1016/j.marenvres.2004.03.047 [doi]
PST - ppublish
SO  - Mar Environ Res. 2004 Aug-Dec;58(2-5):571-5. doi: 
      10.1016/j.marenvres.2004.03.047.