PMID- 15180372
OWN - NLM
STAT- MEDLINE
DCOM- 20040823
LR  - 20190917
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 23
IP  - 5
DP  - 2004 May
TI  - Comparison of two screening bioassays, based on the frog sciatic nerve and yeast 
      cells, for the assessment of herbicide toxicity.
PG  - 1211-8
AB  - Two different test systems, one based on the isolated sciatic nerve of an 
      amphibian and the other on a microbial eukaryote, were used for the assessment of 
      herbicide toxicity. More specifically, we determined the deleterious effects of 
      increasing concentrations of herbicides of different chemical classes 
      (phenoxyacetic acids, triazines, and acetamides), and of 2,4-dichlorophenol 
      (2,4-DCP), a degradation product of the herbicide 2,4-dichlorophenoxyacetic acid 
      (2,4-D), on electrophysiological parameters and the vitality of the axons of the 
      isolated sciatic nerve of the frog (Rana ridibunda) and on the growth curve of 
      the yeast Saccharomyces cerevisiae based on microtiter plate susceptibility 
      assays. The no-observed-effect-concentration (NOEC), defined as the maximum 
      concentration of the tested compound that has no effect on these biological 
      parameters, was estimated. In spite of the different methodological approaches 
      and biological systems compared, the NOEC values were identical and correlated 
      with the lipophilicity of the tested compounds. The relative toxicity established 
      here, 2,4-DCP > alachlor, metolachlor >> metribuzin > 2,4-D, 
      2-methyl-4-chlorophenoxyacetic acid (MCPA), correlates with the toxicity indexes 
      reported in the literature for freshwater organisms. Based on these results, we 
      suggest that the relatively simple, rapid, and low-cost test systems examined 
      here may be of interest as alternative or complementary tests for toxicological 
      assessment of herbicides.
FAU - Papaefthimiou, Chrisovalantis
AU  - Papaefthimiou C
AD  - Laboratory of Animal Physiology, Department of Zoology, School of Biology, 
      Aristotle University, Thessaloniki 54124, Hellas, Greece.
FAU - Cabral, Maria de Guadalupe
AU  - Cabral Mde G
FAU - Mixailidou, Christina
AU  - Mixailidou C
FAU - Viegas, Cristina A
AU  - Viegas CA
FAU - Sa-Correia, Isabel
AU  - Sa-Correia I
FAU - Theophilidis, George
AU  - Theophilidis G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Acetamides)
RN  - 0 (Chlorophenols)
RN  - 0 (Herbicides)
RN  - 0 (Triazines)
RN  - 24S2S61PXL (alachlor)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - QO836138OV (metribuzin)
RN  - R669TG1950 (2,4-dichlorophenol)
RN  - X0I01K05X2 (metolachlor)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/toxicity
MH  - Acetamides/toxicity
MH  - Animals
MH  - Anura
MH  - Biological Assay/*methods
MH  - Chlorophenols/toxicity
MH  - Herbicides/*toxicity
MH  - Saccharomyces cerevisiae/cytology/growth & development
MH  - Sciatic Nerve/*drug effects/metabolism
MH  - Time Factors
MH  - Triazines/toxicity
MH  - Yeasts/cytology/*drug effects/growth & development
EDAT- 2004/06/08 05:00
MHDA- 2004/08/24 05:00
CRDT- 2004/06/08 05:00
PHST- 2004/06/08 05:00 [pubmed]
PHST- 2004/08/24 05:00 [medline]
PHST- 2004/06/08 05:00 [entrez]
AID - 10.1897/03-48 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2004 May;23(5):1211-8. doi: 10.1897/03-48.