PMID- 15180548
OWN - NLM
STAT- MEDLINE
DCOM- 20041215
LR  - 20190917
IS  - 1389-2010 (Print)
IS  - 1389-2010 (Linking)
VI  - 5
IP  - 3
DP  - 2004 Jun
TI  - Single-molecule spectroscopy for nucleic acid analysis: a new approach for 
      disease detection and genomic analysis.
PG  - 271-8
AB  - Recently developed single-molecule spectroscopy (SMS) permits the analysis of 
      fluorescent mixtures one molecule at a time. SMS methods provide the means to 
      make rapid measurements on small, complex samples without the need for 
      separations and target amplification enabling a new class of ultrasensitive 
      nucleic acid assays. Here we give a brief overview of the current state of the 
      art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on 
      two-color single-molecule fluorescence detection of specific nucleic acid 
      sequences. In the future, two-color SMS nucleic acid assays will be used for a 
      variety of applications including: gene expression analysis, disease detection 
      and genomics.
FAU - Goodwin, Peter M
AU  - Goodwin PM
AD  - Los Alamos National Laboratory, Bioscience Division, New Mexico 87545, USA. 
      pmg@lanl.gov
FAU - Nolan, Rhiannon L
AU  - Nolan RL
FAU - Cai, Hong
AU  - Cai H
LA  - eng
GR  - RR01315/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - Netherlands
TA  - Curr Pharm Biotechnol
JT  - Current pharmaceutical biotechnology
JID - 100960530
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*analysis/*chemistry
MH  - Fluorescence Resonance Energy Transfer/instrumentation/*methods
MH  - Gene Expression Profiling/instrumentation/*methods
MH  - Genetic Testing/*methods
MH  - Genomics/instrumentation/methods
MH  - Haplotypes
MH  - Microscopy, Fluorescence, Multiphoton/instrumentation/methods
MH  - Molecular Biology/instrumentation/methods
MH  - Oligonucleotide Array Sequence Analysis/instrumentation/*methods
MH  - Sequence Analysis, DNA/instrumentation/*methods
MH  - Spectrometry, Fluorescence/instrumentation/methods
RF  - 60
EDAT- 2004/06/08 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/06/08 05:00
PHST- 2004/06/08 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/06/08 05:00 [entrez]
AID - 10.2174/1389201043376814 [doi]
PST - ppublish
SO  - Curr Pharm Biotechnol. 2004 Jun;5(3):271-8. doi: 10.2174/1389201043376814.