PMID- 15181271
OWN - NLM
STAT- MEDLINE
DCOM- 20041208
LR  - 20181113
IS  - 0257-277X (Print)
IS  - 0257-277X (Linking)
VI  - 29
IP  - 1-3
DP  - 2004
TI  - Tracking dendritic cells in vivo: insights into DC biology and function.
PG  - 69-80
AB  - Dendritic cells (DCs) play a vital role in the generation of immune responses, 
      participating both in innate immunity as well as in the initiation of adaptive 
      immunity. However, study of this rare cell population in vivo has been hampered 
      by their low frequency as well as by inadequate means to track antigen-bearing 
      DCs. Our laboratory has developed a novel strategy to genetically tag these DCs 
      in the skin, and to monitor their migration from the periphery to the draining 
      lymph nodes. These studies have provided new insights into the frequency of DC 
      migration, the longevity of DCs in the lymphoid organs, as well as the ability of 
      these DCs to function as antigen-presenting cells. Furthermore, the potential 
      applications of this technique include the ability to evaluate DC function after 
      silencing of specific genes.
FAU - Hon, Huiming
AU  - Hon H
AD  - Emory Vaccine Center, Emory University, 950 Gatewood Road NE, Atlanta, GA 30329, 
      USA.
FAU - Jacob, Joshy
AU  - Jacob J
LA  - eng
GR  - T32 GM008169/GM/NIGMS NIH HHS/United States
GR  - R01 AI 52475 1/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - United States
TA  - Immunol Res
JT  - Immunologic research
JID - 8611087
RN  - 0 (Biomarkers)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Biolistics/methods
MH  - Biomarkers/analysis
MH  - Cell Differentiation
MH  - *Cell Movement
MH  - Gene Expression
MH  - Genes, Reporter/genetics
MH  - Integrases/genetics/metabolism
MH  - Langerhans Cells/*immunology/physiology
MH  - Lymph Nodes/metabolism
MH  - Mice
MH  - Skin Physiological Phenomena/immunology
MH  - Viral Proteins/genetics/metabolism
MH  - beta-Galactosidase/analysis/genetics
RF  - 47
EDAT- 2004/06/08 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/06/08 05:00
PHST- 2004/06/08 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/06/08 05:00 [entrez]
AID - IR:29:1-3:069 [pii]
AID - 10.1385/IR:29:1-3:069 [doi]
PST - ppublish
SO  - Immunol Res. 2004;29(1-3):69-80. doi: 10.1385/IR:29:1-3:069.