PMID- 15181456
OWN - NLM
STAT- MEDLINE
DCOM- 20051020
LR  - 20040917
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 11
IP  - 10
DP  - 2004 Oct
TI  - Galectin-1beta, a natural monomeric form of galectin-1 lacking its six 
      amino-terminal residues promotes axonal regeneration but not cell death.
PG  - 1076-83
AB  - We previously identified a novel N-terminally processed form of galectin-1, 
      galectin-1beta (Gal-1beta) whose expression was induced by DeltaFosB. In the 
      present study, the biochemical properties and biological functions of Gal-1beta 
      were compared with the full-length form of galectin-1 (Gal-1alpha). We first 
      purified recombinant mouse Gal-1alpha and beta (rmGal-1alpha, beta) to near 
      homogeneity. The rmGal-1alpha exists as a monomer under oxidized conditions and 
      forms a dimer under reduced conditions, while the rmGal-1beta exists as a monomer 
      regardless of redox conditions. The affinity of rmGal-1beta to beta-lactose was 
      approximately two-fold lower than that of rmGal-1alpha under reduced conditions. 
      The viability of Jurkat cells efficiently decreased when they were exposed to 
      rmGal-1alpha, however, rmGal-1beta barely induced such a reduction. In contrast, 
      both rmGal-1alpha and rmGal-1beta exhibited an equivalent capacity to promote 
      axonal regeneration from the dorsal root ganglion explants. Our results suggest 
      that the biochemical properties of rmGal-1beta determine its biological 
      functions.
FAU - Miura, T
AU  - Miura T
AD  - Division of Neurofunctional Genomics, Department of Immunobiology and 
      Neuroscience, Medical Institute of Bioregulation, Kyushu University, Maidashi 
      Higashi-ku, Fukuoka, Japan.
FAU - Takahashi, M
AU  - Takahashi M
FAU - Horie, H
AU  - Horie H
FAU - Kurushima, H
AU  - Kurushima H
FAU - Tsuchimoto, D
AU  - Tsuchimoto D
FAU - Sakumi, K
AU  - Sakumi K
FAU - Nakabeppu, Y
AU  - Nakabeppu Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (Galectin 1)
RN  - 0 (Lectins)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects/*metabolism
MH  - Cell Death/drug effects
MH  - Circular Dichroism
MH  - Dimerization
MH  - Galectin 1/*chemistry/genetics/metabolism/*pharmacology
MH  - Ganglia, Spinal/cytology/drug effects/metabolism
MH  - Humans
MH  - Jurkat Cells
MH  - Lectins/pharmacology
MH  - Mice
MH  - Models, Molecular
MH  - Nerve Regeneration/*drug effects
MH  - Oxidation-Reduction
MH  - Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Structure-Activity Relationship
EDAT- 2004/06/08 05:00
MHDA- 2005/10/21 09:00
CRDT- 2004/06/08 05:00
PHST- 2004/06/08 05:00 [pubmed]
PHST- 2005/10/21 09:00 [medline]
PHST- 2004/06/08 05:00 [entrez]
AID - 4401462 [pii]
AID - 10.1038/sj.cdd.4401462 [doi]
PST - ppublish
SO  - Cell Death Differ. 2004 Oct;11(10):1076-83. doi: 10.1038/sj.cdd.4401462.