PMID- 15182300
OWN - NLM
STAT- MEDLINE
DCOM- 20040802
LR  - 20131121
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 19
IP  - 11
DP  - 2004 Jun
TI  - Irreversible loss of a subpopulation of cortical interneurons in the absence of 
      glutamatergic network activity.
PG  - 2931-43
AB  - In the cerebral cortex of mammals, gamma-aminobutyric acid (GABA)ergic neurons 
      represent 15-25% of all neurons, depending on the species and area being 
      examined. Because converging evidence suggests that activity may play an 
      important role in the neuritic maturation and synaptic function of GABAergic 
      neurons, it is feasible that activity plays a role in the regulation of the 
      proportion of GABAergic neurons. Here we provide direct evidence that early in 
      cortical development activity blockade may deplete the network of a subpopulation 
      of GABA immunoreactive neurons characterized by their small size and late 
      generation in vitro. In a period of time coinciding with the emergence of 
      synchronous network activity, the survival and morphological differentiation of 
      GABAergic neurons was influenced by long-term blockade of synaptic activity. 
      While GABA(A) receptor antagonists had a minor promoting effect on interneuronal 
      survival during the second week in vitro, antagonists of ionotropic glutamate 
      receptors strongly impaired survival and differentiation of immature GABAergic 
      interneurons. Interneuronal loss was more severe when N-methyl-D-aspartate 
      receptors were blocked than after blockade of 
      alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA)/kainate 
      receptors. The decrease in the density of GABAergic neurons was irreversible, but 
      could be prevented by the simultaneous addition of brain-derived neurotrophic 
      factor (BDNF). These results suggest that there is a narrow time window during 
      neocortical development when glutamatergic activity, and specially NMDA receptor 
      stimulation, is crucial to assure survival and maturation of a subpopulation of 
      late developing GABAergic interneurons.
FAU - de Lima, Ana Dolabela
AU  - de Lima AD
AD  - Otto-von-Guericke Universitat, Medizinische Fakultat, Institut fur Physiologie, 
      39120 Magdeburg, Germany. ana.delima@medizin.uni-magdeburg.de
FAU - Opitz, Thoralf
AU  - Opitz T
FAU - Voigt, Thomas
AU  - Voigt T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Calbindin 2)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (GABA Antagonists)
RN  - 0 (Parvalbumins)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 124-87-8 (Picrotoxin)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 40709-69-1 (bicuculline methiodide)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 76326-31-3 (2-amino-5-phosphopentanoic acid)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
RN  - HG18B9YRS7 (Valine)
RN  - SY7Q814VUP (Calcium)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Age Factors
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Bicuculline/*analogs & derivatives/pharmacology
MH  - Calbindin 2
MH  - Calcium/metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Cell Count/methods
MH  - Cell Size/drug effects/physiology
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Drug Interactions
MH  - Embryo, Mammalian
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - GABA Antagonists/pharmacology
MH  - Glutamic Acid/*metabolism
MH  - Immunohistochemistry/methods
MH  - In Situ Nick-End Labeling/methods
MH  - Interneurons/classification/*cytology/metabolism
MH  - Nerve Net/cytology/*metabolism
MH  - Parvalbumins/metabolism
MH  - Picrotoxin/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Time Factors
MH  - Valine/*analogs & derivatives/pharmacology
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 2004/06/09 05:00
MHDA- 2004/08/03 05:00
CRDT- 2004/06/09 05:00
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2004/08/03 05:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
AID - EJN3403 [pii]
AID - 10.1111/j.0953-816X.2004.03403.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2004 Jun;19(11):2931-43. doi: 10.1111/j.0953-816X.2004.03403.x.