PMID- 15182478
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20131121
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 38
IP  - 3
DP  - 2004 May
TI  - [Studies on the effects of lead on the growth and differentiation of hippocampal 
      neural cells as well as the expression of Oct-2].
PG  - 151-4
AB  - OBJECTIVE: In order to explore the effects of lead on the growth and development 
      of cultured hippocampal neural cells and on the expression of Oct-2, the II 
      subtype POU domain protein. METHODS: Experiment cell model was established using 
      primary culture of hippocampal neural cells from SD rat embryos. Target cells 
      were exposed to lead acetate in the different concentrations, i.e. 10(-1), 10(0), 
      10(1), 10(2), 10(3) micromol/L, while the control group was given the same 
      quantity of the culture medium. The immunohistochemistry method was utilized to 
      detect the expressions of Neurofilament (NF) and Glial Fibrillary Acidic Protein 
      (GFAP), the markers for neuron and astrocyte, respectively, and the expression of 
      Oct-2 as well. RESULTS: The results showed that 10 micromol/L lead acetate 
      treatment caused diminishing of neuronal cell body and the decreases of both axon 
      lengths and inter-cellular connections. In addition, 1 micromol/L lead acetate 
      significantly increased the number of GFAP-positive cells compared with the 
      control group (P < 0.05). By image analysis system, 1 micromol/L lead acetate 
      treatment was found to induce a statistically significant increase of the 
      positive area rate concerning Oct-2 expression in hippocampal neurons and 
      astrocytes, while both positive area rate and integral density of light of Oct-2 
      expression were found to increase markedly in the groups treated by 10 micromol/L 
      lead acetate (P < 0.01). CONCLUSIONS: Lead acetate treatment may contribute to 
      the inhibitions of both growth and differentiation of hippocampus neurons, and to 
      the stimulation of glial cell hyperplasia simultaneously. In addition, the CNS 
      impairments caused by lead is partly correlated with the enhancement of Oct-2 
      expression.
FAU - Chen, Jun
AU  - Chen J
AD  - Department of Occupational and Environmental Health, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, China.
FAU - Zhu, Wei-guo
AU  - Zhu WG
FAU - Chen, Qiu-sheng
AU  - Chen QS
FAU - Lu, Ling
AU  - Lu L
FAU - Chen, Xue-min
AU  - Chen XM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Octamer Transcription Factor-2)
RN  - 0 (Pou2f2 protein, rat)
RN  - 0 (Transcription Factors)
RN  - 2P299V784P (Lead)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/*biosynthesis/genetics
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Mammalian
MH  - Female
MH  - Glial Fibrillary Acidic Protein/biosynthesis/genetics
MH  - Hippocampus/cytology/*metabolism
MH  - Lead/*toxicity
MH  - Neurofilament Proteins/biosynthesis/genetics
MH  - Neurons/cytology/metabolism
MH  - Octamer Transcription Factor-2
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcription Factors/*biosynthesis/genetics
EDAT- 2004/06/09 05:00
MHDA- 2005/05/25 09:00
CRDT- 2004/06/09 05:00
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2004 May;38(3):151-4.