PMID- 15182730
OWN - NLM
STAT- MEDLINE
DCOM- 20050216
LR  - 20071115
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 4
IP  - 7
DP  - 2004 Jul
TI  - Up-regulation of phospholipase Cgamma1 and phospholipase D during the 
      differentiation of human monocytes to dendritic cells.
PG  - 911-20
AB  - Phospholipase C (PLC)gamma and phospholipase D (PLD) play pivotal roles in the 
      signal transduction required for various cellular responses, including cell 
      proliferation and differentiation. Dendritic cells (DCs), which are professional 
      antigen-presenting cells, can be generated from human monocytes by stimulating 
      the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and 
      interleukin 4 (IL-4). We investigated whether PLCgamma and PLD expression levels 
      can be changed during the differentiation of the human monocytes into DCs. The 
      enzymatic activity and protein level of PLC gamma1 were significantly increased 
      in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC 
      gamma2 were unaltered. Moreover, the enzymatic activity and protein level of 
      PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to 
      DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was 
      found to be correlated with the up-regulations of PLCgamma1 and PLD, and the 
      phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and 
      by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic 
      acid. Thus, suggesting that PLC and PLD participate in the process. This study 
      suggests that the up-regulations of PLCgamma1 and PLD are accompanied by the 
      differentiation of monocytes into DCs, which results in increased phagocytic 
      activity.
CI  - Copyright 2004 Elsevier B.V.
FAU - Kang, Hyun Kyu
AU  - Kang HK
AD  - Medical Research Center for Cancer Molecular Therapy, Dong-A University, Busan 
      602-714, South Korea.
FAU - Lee, Ha Young
AU  - Lee HY
FAU - Lee, Youl-Nam
AU  - Lee YN
FAU - Jo, Eun Jin
AU  - Jo EJ
FAU - Kim, Jung Im
AU  - Kim JI
FAU - Kim, Gi-Young
AU  - Kim GY
FAU - Park, Yeong Min
AU  - Park YM
FAU - Min, Do Sik
AU  - Min DS
FAU - Yano, Akihiko
AU  - Yano A
FAU - Kwak, Jong-Young
AU  - Kwak JY
FAU - Bae, Yoe-Sik
AU  - Bae YS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Glycerophospholipids)
RN  - 0 (phosphatidylbutanol)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - EC 3.1.4.4 (Phospholipase D)
SB  - IM
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Dendritic Cells/*drug effects/enzymology
MH  - Glycerophospholipids/biosynthesis
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-4/pharmacology
MH  - Monocytes/cytology/*drug effects/enzymology
MH  - Phagocytosis/drug effects
MH  - Phospholipase C gamma
MH  - Phospholipase D/*biosynthesis/immunology
MH  - Signal Transduction
MH  - Type C Phospholipases/*biosynthesis/immunology
MH  - Up-Regulation
EDAT- 2004/06/09 05:00
MHDA- 2005/02/17 09:00
CRDT- 2004/06/09 05:00
PHST- 2004/03/08 00:00 [received]
PHST- 2004/03/30 00:00 [revised]
PHST- 2004/04/01 00:00 [accepted]
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2005/02/17 09:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
AID - S1567-5769(04)00119-5 [pii]
AID - 10.1016/j.intimp.2004.04.001 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2004 Jul;4(7):911-20. doi: 10.1016/j.intimp.2004.04.001.