PMID- 15183092
OWN - NLM
STAT- MEDLINE
DCOM- 20040810
LR  - 20081121
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 288
IP  - 1-2
DP  - 2004 May
TI  - ELISPOT cell rescue.
PG  - 135-47
AB  - The enzyme-linked immunospot (ELISPOT) assay is a highly sensitive and 
      reproducible method for quantifying T cell-mediated immune responses, and has 
      been used to measure antigen-specific responses post-vaccination. While there are 
      several advantages of the ELISPOT assay for use in field settings for large-scale 
      vaccination trials, blood draw volumes are often limited, and the number of 
      antigen-specific responses that can be measured is constrained by the limited 
      cell number. We reasoned that it should be possible to salvage and rescue viable 
      cells from a completed ELISPOT assay post-incubation, to use for further 
      experimentation. Here, we show that cells rescued from an ELISPOT plate after 
      assay are viable, and may be used in a second cytokine-producing assay, in a 
      proliferation assay, or to provide a source of DNA for genetic studies such as 
      human leukocyte antigen (HLA) typing. Rescue of cells after an ELISPOT assay will 
      be particularly useful for increasing sample utility and maximizing data 
      collection from T cell assays in vaccine trials.
CI  - Copyright 2004 Elsevier B.V.
FAU - Meiklejohn, Duncan A
AU  - Meiklejohn DA
AD  - Gladstone Institute of Virology and Immunology, University of California, PO Box 
      419100, San Francisco, CA 94141-9100, USA.
FAU - Karlsson, R Karl
AU  - Karlsson RK
FAU - Karlsson, Annika C
AU  - Karlsson AC
FAU - Chapman, Joan M
AU  - Chapman JM
FAU - Nixon, Douglas F
AU  - Nixon DF
FAU - Schweighardt, Becky
AU  - Schweighardt B
LA  - eng
GR  - AI46254/AI/NIAID NIH HHS/United States
GR  - PA01158/PHS HHS/United States
GR  - U19AI51596/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (HLA Antigens)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*analysis
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - HLA Antigens/*genetics/immunology
MH  - Humans
MH  - Interferon-gamma/analysis/immunology
MH  - Leukocytes/*immunology/metabolism
EDAT- 2004/06/09 05:00
MHDA- 2004/08/11 05:00
CRDT- 2004/06/09 05:00
PHST- 2003/10/20 00:00 [received]
PHST- 2004/02/27 00:00 [revised]
PHST- 2004/03/21 00:00 [accepted]
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2004/08/11 05:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
AID - S0022175904001139 [pii]
AID - 10.1016/j.jim.2004.03.006 [doi]
PST - ppublish
SO  - J Immunol Methods. 2004 May;288(1-2):135-47. doi: 10.1016/j.jim.2004.03.006.