PMID- 15183442
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20220321
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 22
IP  - 4
DP  - 2004 Jul
TI  - Comparison of the response of primary human blood monocytes and the U937 human 
      monocytic cell line to two different sizes of alumina ceramic particles.
PG  - 832-8
AB  - It is well recognized that wear particles derived from orthopaedic implants have 
      the potential to induce inflammation, which may eventually lead to aseptic 
      loosening of the artificial joint. We hypothesized that alumina ceramic particles 
      of different sizes cause a differential cytokine response by human monocytes. To 
      test this hypothesis a human monocytic cell line (U937) and primary human blood 
      monocytes obtained from healthy volunteers were exposed to ceramic particles 
      within the range known to be generated in vivo. Cellular responses were measured 
      by quantifying the relative gene expression of 12 different cytokines using 
      TAQman Real-Time Polymerase Chain Reaction (RT-PCR). Our results demonstrate that 
      at a particle to cell ratio of 100:1, 0.5 microm ceramic particles consistently 
      provoked higher amounts of Interleukin-1alpha (IL-1alpha), IL-1beta, IL-8, IL-10 
      and Tumor necrosis factor-alpha (TNF-alpha) steady state mRNA by U937 cells. As 
      expected, the variability of cytokine expression in primary blood monocytes was 
      much higher compared to the cell line however, a similar trend was observed. 
      These results show a differential response to ceramic particle size, which may 
      imply that 0.5 microm particles are less biocompatible. New ceramic implants can 
      be designed to generate a known particle size range in vivo. Implant materials of 
      this type may induce relatively lower levels of production of inflammatory 
      cytokines resulting in a reduced incidence of failure due to aseptic loosening.
FAU - Yagil-Kelmer, Efrat
AU  - Yagil-Kelmer E
AD  - Department of Veterinary Pathobiology, University of Missouri, 1600 East Rollins 
      Road, Columbia, MO 65211, USA.
FAU - Kazmier, Peter
AU  - Kazmier P
FAU - Rahaman, Mohamed N
AU  - Rahaman MN
FAU - Bal, B Sonny
AU  - Bal BS
FAU - Tessman, Ronald K
AU  - Tessman RK
FAU - Estes, D Mark
AU  - Estes DM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - CPD4NFA903 (Aluminum)
SB  - IM
MH  - Aluminum/metabolism/*pharmacology
MH  - Biocompatible Materials/metabolism/*pharmacology
MH  - Cell Survival/drug effects
MH  - Ceramics/metabolism/*pharmacology
MH  - Cytokines/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Monocytes/*drug effects/metabolism
MH  - Particle Size
MH  - Phagocytosis/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - U937 Cells/*drug effects/metabolism
EDAT- 2004/06/09 05:00
MHDA- 2004/07/21 05:00
CRDT- 2004/06/09 05:00
PHST- 2003/04/17 00:00 [received]
PHST- 2003/10/31 00:00 [accepted]
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
AID - S0736026603002845 [pii]
AID - 10.1016/j.orthres.2003.10.022 [doi]
PST - ppublish
SO  - J Orthop Res. 2004 Jul;22(4):832-8. doi: 10.1016/j.orthres.2003.10.022.