PMID- 15184078
OWN - NLM
STAT- MEDLINE
DCOM- 20040730
LR  - 20121115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 319
IP  - 3
DP  - 2004 Jul 2
TI  - Triptolide (PG-490) induces apoptosis of dendritic cells through sequential p38 
      MAP kinase phosphorylation and caspase 3 activation.
PG  - 980-6
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells that play 
      crucial roles in the regulation of immune response. Triptolide, an active 
      component purified from the medicinal plant Tripterygium wilfordii Hook F., has 
      been demonstrated to act as a potent immunosuppressive drug capable of inhibiting 
      T cell activation and proliferation. However, little is known about the effects 
      of triptolide on DCs. The present study shows that triptolide does not affect 
      phenotypic differentiation and LPS-induced maturation of murine DCs. But 
      triptolide can dramatically reduce cell recovery by inducing apoptosis of DCs at 
      concentration as low as 10ng/ml, as demonstrated by phosphatidylserine exposure, 
      mitochondria potential decrease, and nuclear DNA condensation. Triptolide induces 
      activation of p38 in DCs, which precedes the activation of caspase 3. SB203580, a 
      specific kinase inhibitor for p38, can block the activation of caspase 3 and 
      inhibit the resultant apoptosis of DCs. Our results suggest that the 
      anti-inflammatory and immunosuppressive activities of triptolide may be due, in 
      part, to its apoptosis-inducing effects on DCs.
FAU - Liu, Qiuyan
AU  - Liu Q
AD  - Institute of Immunology, Second Military Medical University, 800 Xiangyin Road, 
      Shanghai 200433, PR China.
FAU - Chen, Taoyong
AU  - Chen T
FAU - Chen, Huabiao
AU  - Chen H
FAU - Zhang, Minghui
AU  - Zhang M
FAU - Li, Nan
AU  - Li N
FAU - Lu, Zhanjun
AU  - Lu Z
FAU - Ma, Pengcheng
AU  - Ma P
FAU - Cao, Xuetao
AU  - Cao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Diterpenes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Phenanthrenes)
RN  - 19ALD1S53J (triptolide)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/metabolism
MH  - Diterpenes/*pharmacology
MH  - Enzyme Activation
MH  - Epoxy Compounds
MH  - Immunosuppressive Agents/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Phenanthrenes/*pharmacology
MH  - Phenotype
MH  - Phosphorylation
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2004/06/09 05:00
MHDA- 2004/07/31 05:00
CRDT- 2004/06/09 05:00
PHST- 2004/04/03 00:00 [received]
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2004/07/31 05:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
AID - S0006291X04009404 [pii]
AID - 10.1016/j.bbrc.2004.04.201 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 Jul 2;319(3):980-6. doi: 
      10.1016/j.bbrc.2004.04.201.