PMID- 15184347
OWN - NLM
STAT- MEDLINE
DCOM- 20050126
LR  - 20171116
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 16
IP  - 7
DP  - 2004 Jul
TI  - Dendritic cells generated in the presence of interferon-alpha stimulate 
      allogeneic CD4+ T-cell proliferation: modulation by autocrine IL-10, enhanced 
      T-cell apoptosis and T regulatory type 1 cells.
PG  - 1037-52
AB  - Dendritic cells (DCs) generated in the presence of IFN-alpha (IFN-DCs) exhibit 
      high expression of major histocompatibility and co-stimulatory molecules and a 
      potent ability to stimulate CD8(+) T-cell responses. Here, we found that IFN-DCs 
      were more potent stimulators of bulk and purified CD8(+) T-cell proliferation, as 
      compared with IL-4-DCs. In contrast, IFN-DCs were less efficient than IL-4-DCs in 
      stimulating allogeneic CD4(+) T-cell proliferation, due to a weak induction of 
      naive CD4(+)CD45RO(-) T-cell proliferation by these DCs. However, both DC 
      populations induced similar levels of proliferation of memory CD4(+)CD45RO(+) T 
      cells. IFN-DCs and IL-4-DCs exhibited a similar phenotype and production of IL-10 
      following maturation induced by CD40 ligation. In contrast, IFN-DCs produced 
      higher levels of IL-10 during the first days of differentiation. In addition, 
      neutralization of IL-10 during the differentiation of DCs increased the 
      expression of DC-LAMP and MHC class II by IFN-DCs, and the ability of IFN-DCs to 
      stimulate allogeneic CD4(+) T-cell proliferation at similar levels, than 
      IL-4-DCs. Independently of IL-10 production, IFN-DCs were found to induce higher 
      levels of CD4(+)T-cell apoptosis, this effect being more sticking on naive T 
      cells. Finally, we demonstrated that IFN-DCs induced a differentiation bias of 
      naive CD4(+) T cells towards Th1 and Tr1 cells, compared to IL-4-DCs. Taken 
      together, these results indicate that, despite the induction of Tr1 cells and 
      enhanced apoptosis of naive CD4(+) T cells, IFN-DCs are potent stimulators of 
      CD8(+) and memory CD4(+) T cells, and induce a strong polarization of naive 
      CD4(+) T cells towards Th1 cells, further supporting their use in immune-based 
      therapy.
FAU - Carbonneil, Cedric
AU  - Carbonneil C
AD  - INSERM U430, Institut des Cordeliers, Paris, France.
FAU - Saidi, Hela
AU  - Saidi H
FAU - Donkova-Petrini, Vladimira
AU  - Donkova-Petrini V
FAU - Weiss, Laurence
AU  - Weiss L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040607
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (CD40 Antigens)
RN  - 0 (Interferon-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
SB  - IM
MH  - Apoptosis/*immunology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology
MH  - CD40 Antigens/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Division/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Immunologic Memory/immunology
MH  - Interferon-alpha/*immunology
MH  - Interleukin-10/*immunology
MH  - Interleukin-4/immunology
MH  - Leukocyte Common Antigens/immunology
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 1
MH  - Th1 Cells/immunology
EDAT- 2004/06/09 05:00
MHDA- 2005/01/27 09:00
CRDT- 2004/06/09 05:00
PHST- 2004/06/09 05:00 [pubmed]
PHST- 2005/01/27 09:00 [medline]
PHST- 2004/06/09 05:00 [entrez]
AID - dxh106 [pii]
AID - 10.1093/intimm/dxh106 [doi]
PST - ppublish
SO  - Int Immunol. 2004 Jul;16(7):1037-52. doi: 10.1093/intimm/dxh106. Epub 2004 Jun 7.