PMID- 15186346
OWN - NLM
STAT- MEDLINE
DCOM- 20040921
LR  - 20061115
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 6
IP  - 7
DP  - 2004 Jul
TI  - Design and application of two oligonucleotide probes for the identification of 
      Geodermatophilaceae strains using fluorescence in situ hybridization (FISH).
PG  - 678-85
AB  - Bacteria of the family of Geodermatophilaceae are actively involved in the decay 
      processes [Urzi, C. and Realini, M. (1998) Int Biodeterior Biodegrad 42: 45-54; 
      Urzi, C., Salamone, P., Schumann, P., and Stackebrandt, E. (2000) Int J Syst Evol 
      Microbiol 50: 529-536] of stone monuments. Characterization of isolates includes 
      phenotypic, chemotaxonomic and genetic analysis often requiring long-term 
      procedures. The use of specific probes for members of Geodermatophilaceae family 
      could be useful for the easy detection of those strains colonizing rock surfaces 
      and involved in the biodeterioration. Two 16S rRNA-targeted oligonucleotide 
      probes were designed for the specific detection of members of the family 
      Geodermatophilaceae using fluorescence in situ hybridization (FISH); one probe 
      specific for members of the two genera Geodermatophilus/Blastococcus and the 
      second for members of the genus Modestobacter.
FAU - Urzi, Clara
AU  - Urzi C
AD  - Department of Microbiological, Genetic and Molecular Sciences, University of 
      Messina, Salita Sperone, 31, I-98166 Messina, Italy. Clara.Urzi@unime.it
FAU - La Cono, Violetta
AU  - La Cono V
FAU - Stackebrandt, Erko
AU  - Stackebrandt E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Actinomycetales/*genetics/*isolation & purification
MH  - Environmental Microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - *Oligonucleotide Probes
MH  - RNA, Bacterial/analysis/genetics
MH  - RNA, Ribosomal, 16S/analysis/genetics
MH  - Sensitivity and Specificity
EDAT- 2004/06/10 05:00
MHDA- 2004/09/24 05:00
CRDT- 2004/06/10 05:00
PHST- 2004/06/10 05:00 [pubmed]
PHST- 2004/09/24 05:00 [medline]
PHST- 2004/06/10 05:00 [entrez]
AID - EMI619 [pii]
AID - 10.1111/j.1462-2920.2004.00619.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2004 Jul;6(7):678-85. doi: 10.1111/j.1462-2920.2004.00619.x.