PMID- 15186736
OWN - NLM
STAT- MEDLINE
DCOM- 20041203
LR  - 20061115
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 13
IP  - 2
DP  - 2004 Apr
TI  - Inhibition of cord blood cell expansion by human herpesvirus 6 in vitro.
PG  - 197-203
AB  - To elucidate the role of human herpesvirus 6 (HHV-6) in hematopoiesis, the 
      influence of HHV-6A and HHV-6B on the in vitro expansion and differentiation of 
      cord blood (CB) progenitor cells was investigated in liquid culture. 
      Nonfractionated CB mononuclear cells (CB-MNC) or MACS-enriched CD34+ CB cells 
      were seeded in liquid culture under conditions allowing maximal expansion of 
      nucleated cells. Cells were either incubated with HHV-6A- or HHV-6B-containing 
      cell culture supernatants or a virus-free control. After 7, 14, and 21 days, 
      cells were analyzed for growth by cell count, for differentiation by flow 
      cytometry, and for HHV-6 infection by antigen detection or PCR. Expansion of 
      CB-MNC was significantly inhibited by HHV-6A and HHV-6B for a period of 3 weeks, 
      including reduced proportions of CD34+ and CD33+ cells in HHV-6-treated cultures 
      on day 7. In contrast, when starting with enriched CD34+ cells, the expansion was 
      only affected by HHV-6A. Inhibition of CD34 and CD33 cell development was less 
      pronounced in these cultures compared to CB-MNC cultures. However, HHV-6 antigen 
      and DNA was detectable in these cultures. In conclusion, although HHV-6A 
      inhibited expansion of CD34 progenitor cells, HHV-6B inhibited growth of immature 
      CB cells only in interaction with nonfractionated CB-MNC.
CI  - Copyright Mary Ann Liebert, Inc.
FAU - Nitsche, Andreas
AU  - Nitsche A
AD  - Medizinische Klinik II m.S. Onkologie und Hamatologie, Charite, Humboldt 
      Universitat, Berlin, Germany.
FAU - Fleischmann, Jessika
AU  - Fleischmann J
FAU - Klima, Kai-Michael
AU  - Klima KM
FAU - Radonic, Aleksandar
AU  - Radonic A
FAU - Thulke, Stefanie
AU  - Thulke S
FAU - Siegert, Wolfgang
AU  - Siegert W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Antigens, CD34/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Fetal Blood/*cytology/metabolism/*virology
MH  - Hematopoietic Stem Cells/cytology/metabolism/virology
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Leukocyte Count
MH  - Leukocytes, Mononuclear/cytology/metabolism/*virology
EDAT- 2004/06/10 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/06/10 05:00
PHST- 2004/06/10 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/06/10 05:00 [entrez]
AID - 10.1089/154732804323046800 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2004 Apr;13(2):197-203. doi: 10.1089/154732804323046800.