PMID- 15187581
OWN - NLM
STAT- MEDLINE
DCOM- 20041208
LR  - 20181130
IS  - 0955-8810 (Print)
IS  - 0955-8810 (Linking)
VI  - 15
IP  - 3
DP  - 2004 May
TI  - Central injection of botulinum neurotoxins: behavioural effects in mice.
PG  - 233-40
AB  - Strains of Clostridium botulinum produce seven antigenically distinct botulinum 
      neurotoxins (BoNTs) designated as serotypes A-G. All serotypes interfere with 
      neural transmission by blocking the release of acetylcholine in cholinergic 
      neurons. They cleave specific sites on proteins of the SNARE [soluble 
      n-ethylmaleimide-sensitive factor (NSF) attachment protein receptor] complex, 
      which play a key role in neuroexocytosis. This study assessed the behavioural 
      effects due to central administration of BoNTs in mice. CD1 mice were injected 
      intracerebroventricularly (icv) with sub-lethal doses of BoNT/A or /B and their 
      behavioural responses in conditioning of active avoidance, object recognition 
      test and pharmacologically induced locomotor activity were tested. Compared to 
      control mice, BoNT-treated mice showed: (1) a reduced capacity to discriminate a 
      novel object within a familiar environment; (2) an enhanced stimulant effect by 
      scopolamine and a depressant effect by oxotremorine on locomotor activity. In 
      contrast, central injection of BoNTs did not alter active avoidance acquisition. 
      These results suggest an in vivo functional alteration due to the action of BoNTs 
      directly administered into the central nervous system. The present data 
      demonstrate that BoNTs may represent an analytical tool for studying the 
      functional role of cholinergic neurons.
FAU - Luvisetto, S
AU  - Luvisetto S
AD  - Istituto di Neuroscienze del CNR, Sez. di Psicobiologia e Psicofarmacologia, 
      Roma, Italy. s.luvisetto@ipsifar.rm.cnr.it
FAU - Marinelli, S
AU  - Marinelli S
FAU - Rossetto, O
AU  - Rossetto O
FAU - Montecucco, C
AU  - Montecucco C
FAU - Pavone, F
AU  - Pavone F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Behav Pharmacol
JT  - Behavioural pharmacology
JID - 9013016
RN  - 0 (Cholinergic Agents)
RN  - 0Y70779M1F (rimabotulinumtoxinB)
RN  - 5RY0UWH1JL (Oxotremorine)
RN  - DL48G20X8X (Scopolamine)
RN  - EC 3.4.24.69 (Botulinum Toxins)
RN  - EC 3.4.24.69 (Botulinum Toxins, Type A)
SB  - IM
MH  - Animals
MH  - Avoidance Learning/drug effects
MH  - Behavior, Animal/*drug effects
MH  - Botulinum Toxins/*pharmacology
MH  - Botulinum Toxins, Type A/*pharmacology
MH  - Brain/drug effects
MH  - Cholinergic Agents/*pharmacology
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Motor Activity/drug effects
MH  - Oxotremorine/pharmacology
MH  - Scopolamine/pharmacology
MH  - Time Factors
EDAT- 2004/06/10 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/06/10 05:00
PHST- 2004/06/10 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/06/10 05:00 [entrez]
AID - 00008877-200405000-00007 [pii]
PST - ppublish
SO  - Behav Pharmacol. 2004 May;15(3):233-40.