PMID- 15188138
OWN - NLM
STAT- MEDLINE
DCOM- 20040716
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 35
IP  - 6
DP  - 2004 Jun
TI  - Expression of interleukin-18 by nasopharyngeal carcinoma cells: a factor that 
      possibly initiates the massive leukocyte infiltration.
PG  - 722-8
AB  - Interleukin-18 (IL-18) is a single-chain cytokine that is produced by various 
      cells. With interleukin-12 (IL-12), it synergistically stimulates activated T 
      cells and natural killer (NK) cells to produce interferon-gamma (IFN-gamma). 
      Nasopharyngeal carcinoma (NPC) is the most common form of nasal and 
      nasopharyngeal malignancy, and in NPC tumor tissues there is an intense leukocyte 
      infiltration comprising predominantly T cells and macrophages. We previously 
      showed an increased expression of IFN-gamma in the infiltrating T cells. To 
      identify the cells that provide IL-12 and IL-18 for stimulating the expression of 
      IFN-gamma in activated T cells, NPC cell lines CNE-2 and HK-1, as well as 
      biopsies obtained from NPC and control individuals, were examined. CNE-2 and HK-1 
      cells were found to express messenger RNA encoding IL-18, but not IL-12. Secreted 
      IL-18 was detected in the culture supernatant. Addition of a caspase-1 inhibitor 
      decreased the secretion level, indicating that this IL-18 secretion was caspase-1 
      dependent. Moreover, the in vitro IL-18 production in NPC cell lines correlated 
      with the NPC tumor cells in situ. NPC tumor cells in the biopsies produced IL-18, 
      as detected by immunohistochemistry and immunofluorescent double staining. In 
      contrast, IL-18 expression was not observed in the control biopsies. We suggest 
      that IL-18 secreted by NPC tumor cells plays a role in initiating the leukocyte 
      infiltration process. IL-18 stimulates T cells and NK cells to produce IFN-gamma, 
      which consequently activates macrophages and other immune cells to secrete 
      chemokines to start a leukocyte recruitment cascade.
FAU - Hu, Huaizhong
AU  - Hu H
AD  - Department of Microbiology, Faculty of Medicine, National University Medical 
      Institute, National University of Singapore, Singapore, Singapore.
FAU - Tang, Kin Fai
AU  - Tang KF
FAU - Chua, Yen Ni
AU  - Chua YN
FAU - Lu, Jinhua
AU  - Lu J
FAU - Feng, Ping
AU  - Feng P
FAU - Chew, Chuan Tieh
AU  - Chew CT
FAU - Chan, Soh Ha
AU  - Chan SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Interleukin-18)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Caspase 1/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-18/*biosynthesis
MH  - Leukocytes/*metabolism
MH  - Nasopharyngeal Neoplasms/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/06/10 05:00
MHDA- 2004/07/17 05:00
CRDT- 2004/06/10 05:00
PHST- 2004/06/10 05:00 [pubmed]
PHST- 2004/07/17 05:00 [medline]
PHST- 2004/06/10 05:00 [entrez]
AID - S0046817704001091 [pii]
AID - 10.1016/j.humpath.2004.01.026 [doi]
PST - ppublish
SO  - Hum Pathol. 2004 Jun;35(6):722-8. doi: 10.1016/j.humpath.2004.01.026.