PMID- 15188143
OWN - NLM
STAT- MEDLINE
DCOM- 20040716
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 35
IP  - 6
DP  - 2004 Jun
TI  - Determination of DNA ploidy by fluorescence in situ hybridization (FISH) in 
      hydatidiform moles: evaluation of FISH on isolated nuclei.
PG  - 752-8
AB  - In the past 20 years, the diagnosis of hydatidiform moles has become more 
      difficult because of the widespread use of early uterine evacuation. 
      Differentiating hydropic degeneration, partial, and complete moles is important 
      because of their different prognosis. However, clinical diagnosis is less 
      obvious, and the pathologist has to separate the different entities on the basis 
      of very subtle morphologic criteria. In difficult cases, ploidy may be determined 
      by various methods, including fluorescence in situ hybridization (FISH) on 
      routine histological sections from paraffin-embedded specimens. However, FISH 
      analysis is often difficult because of the presence of numerous truncated nuclei. 
      In this context, we have tested the advantages of FISH on isolated nuclei, a 
      well-known variant of the technique that might be more sensitive. We reviewed 24 
      cases of products of abortion: hydropic degenerations, complete hydatidiform 
      moles, partial moles, and nonmolar triploidies. After histological review, FISH 
      on isolated nuclei proved conclusive in all cases. The results could be easily 
      interpreted thanks to the reduced number of truncated nuclei. The percentage of 
      cells with 2 signals was always >70% in the diploid cases and >60% in the 
      triploid cases. In conclusion, this sensitive technique seems to be a valuable 
      tool for the diagnosis of moles.
FAU - Yver, Matthieu
AU  - Yver M
AD  - Pathology Department, Hopital Pellegrin, and the Histology and Embryology 
      Laboratory, UMR Centre National de la Recherche Scientifique 5541, V. Segalan 
      University, Bordeaux, France.
FAU - Carles, Dominique
AU  - Carles D
FAU - Bloch, Bertrand
AU  - Bloch B
FAU - Bioulac-Sage, Paulette
AU  - Bioulac-Sage P
FAU - Martin Negrier, Marie-Laure
AU  - Martin Negrier ML
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
SB  - IM
MH  - Cell Nucleus/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Hydatidiform Mole/*diagnosis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Ploidies
MH  - Pregnancy
MH  - Pregnancy Complications
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Uterine Neoplasms/*diagnosis/genetics
EDAT- 2004/06/10 05:00
MHDA- 2004/07/17 05:00
CRDT- 2004/06/10 05:00
PHST- 2004/06/10 05:00 [pubmed]
PHST- 2004/07/17 05:00 [medline]
PHST- 2004/06/10 05:00 [entrez]
AID - S0046817704000747 [pii]
AID - 10.1016/j.humpath.2004.01.020 [doi]
PST - ppublish
SO  - Hum Pathol. 2004 Jun;35(6):752-8. doi: 10.1016/j.humpath.2004.01.020.