PMID- 15188657
OWN - NLM
STAT- MEDLINE
DCOM- 20040809
LR  - 20161109
IS  - 0507-4088 (Print)
IS  - 0507-4088 (Linking)
VI  - 49
IP  - 3
DP  - 2004 May-Jun
TI  - [Analysis of genome determinants of virulence and setting-up of a library of 
      full-size genome copies of the attenuated virus strain of the porcine 
      reproductive and respiratory syndrome virus North American type].
PG  - 56-63
AB  - Primary genome structures of 3 variants of the NADC-8 North American virulent 
      strain of porcine reproductive and respiratory syndrome virus (PRRSV) were 
      compared for the purpose of detecting any potential genetic virulence 
      determinants of genus Arterivirus. Apart from the virulent variant, we also 
      investigated the attenuated variant, obtained after 251 passages in cell culture, 
      and the intermediate variant isolated from a pig after a partial reversion of the 
      attenuated virus. The attenuated variant genome acquired a 3-nucleotide deletion 
      and 50 mutations versus its virulent precursor. A comparison of the attenuated 
      and intermediary virus variants denoted 8 nucleotide mutations entailing 
      substitutions of 6 amino acids in 3 open reading frames (ORF1a, ORF1b and ORF6). 
      A 32-clone library was constructed in the pACYC177 plasmid vector, which 
      comprised full-size copies of the genome of the NADC-8 attenuated variant strain 
      (251), virus PPCC, for the purpose of experimentally verifying the functional 
      role of the obtained mutations. Full-size analogues ((+)-chain of RNA) of the 
      viral genome, comprising the CAP-structures and polyadenylated ones were obtained 
      in vitro on the basis of the cloned DNA. Seven of the 8 analyzed clones of the 
      viral genome were infected and their insertion into the MARC-145 cell resulted in 
      obtaining of infectious PRRSVs. Four of the constructed recombinant viruses had 
      delayed growth parameters, and 3 of them were similar to the parental strain. The 
      described technology (inverse genetics) would make it possible to introduce 
      changes into the viral genome in applied and fundamental research of 
      Arteriviruses.
FAU - Grebennikova, T V
AU  - Grebennikova TV
FAU - Zaberezhnyi, A D
AU  - Zaberezhnyi AD
FAU - Musienko, M I
AU  - Musienko MI
FAU - Mengeling, W
AU  - Mengeling W
FAU - Lager, K
AU  - Lager K
FAU - Aliper, T I
AU  - Aliper TI
FAU - Nepoklonov, E A
AU  - Nepoklonov EA
LA  - rus
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Analiz genomnykh determinant virulentnosti i sozdanie biblioteki polnorazmernykh 
      infektsionnykh kopii genoma attenuirovannogo shtamma virusa 
      reproduktivno-repriratornogo sindroma svinei severoamerikanskogo tipa.
PL  - Russia (Federation)
TA  - Vopr Virusol
JT  - Voprosy virusologii
JID - 0417337
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Genetic Variation
MH  - *Genome, Viral
MH  - Mutation
MH  - Open Reading Frames
MH  - Plasmids
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/pathogenicity
MH  - Recombination, Genetic
MH  - Swine
MH  - Virulence
EDAT- 2004/06/11 05:00
MHDA- 2004/08/10 05:00
CRDT- 2004/06/11 05:00
PHST- 2004/06/11 05:00 [pubmed]
PHST- 2004/08/10 05:00 [medline]
PHST- 2004/06/11 05:00 [entrez]
PST - ppublish
SO  - Vopr Virusol. 2004 May-Jun;49(3):56-63.