PMID- 15188957
OWN - NLM
STAT- MEDLINE
DCOM- 20041123
LR  - 20220408
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 68
IP  - 2
DP  - 2004 Apr
TI  - An experimental model to evaluate the role of transport vehicles as a source of 
      transmission of porcine reproductive and respiratory syndrome virus to 
      susceptible pigs.
PG  - 128-33
AB  - The objectives of this study were to determine the concentration of porcine 
      reproductive and respiratory syndrome virus (PRRSV) in a scale-model trailer that 
      was required to infect susceptible pigs, evaluate the potential of 
      PRRSV-contaminated transport vehicles to infect naive pigs and assess 4 
      sanitation programs for the prevention of virus spread. To maximize study power, 
      scale models (1:150) of weaned-pig trailers were constructed that provided an 
      animal density equal to that of an actual weaned-pig trailer capable of 
      transporting 300 pigs. The 1st aim involved contaminating the interior of the 
      model trailers with various concentrations (10(1) to 10(4) TCID50/mL) of PRRSV MN 
      30-100, then housing sentinel pigs in the trailers for 2 h. Pigs exposed to 
      trailers contaminated with > or = 10(3) TCID50/mL became infected. The 2nd aim 
      involved housing experimentally infected seeder pigs in trailers for 2 h, then 
      directly introducing sentinel pigs for 2 h. Infection of sentinels was 
      demonstrated in 3 of 4 replicates. The 3rd aim involved applying 1 of 4 
      sanitation procedures (treatments) to contaminated trailers. Treatment 1 
      consisted of manual scraping of the interior to remove soiled bedding (wood 
      chips). Treatment 2 consisted of bedding removal, washing (80 degrees C, 20,500 
      kPa), and disinfecting (with 1:256 phenol; 10-min contact time). Treatment 3 
      consisted of treatment 2, followed by freezing and thawing. Treatment 4 consisted 
      of bedding removal, washing, disinfecting, and drying. Ten replicates were 
      conducted per treatment. Pretreatment swabs from all trailers tested positive by 
      polymerase chain reaction (PCR). Post-treatment swabs were PCR-positive for all 
      trailers except those that were washed, disinfected, and dried. Infection of 
      sentinel pigs by PRRSV was also detected by PCR after all treatments except 
      washing, disinfecting, and drying. Under the conditions of this study, drying 
      appeared to be an important component of a sanitation program for ensuring PRRSV 
      biosecurity of transport vehicles.
FAU - Dee, Scott A
AU  - Dee SA
AD  - University of Minnesota Swine Disease Eradication Center, 385C 1988 Fitch Avenue, 
      St. Paul, Minnesota 55108, USA. deexx004@umn.edu
FAU - Deen, John
AU  - Deen J
FAU - Otake, Satoshi
AU  - Otake S
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
SB  - IM
MH  - Animals
MH  - Disinfection/*methods
MH  - Models, Theoretical
MH  - Polymerase Chain Reaction/veterinary
MH  - Population Density
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Swine
MH  - Swine Diseases/*transmission/virology
MH  - *Transportation
PMC - PMC1142156
EDAT- 2004/06/11 05:00
MHDA- 2004/12/16 09:00
PMCR- 2004/07/01
CRDT- 2004/06/11 05:00
PHST- 2004/06/11 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/06/11 05:00 [entrez]
PHST- 2004/07/01 00:00 [pmc-release]
AID - pg128 [pii]
PST - ppublish
SO  - Can J Vet Res. 2004 Apr;68(2):128-33.