PMID- 15189295 OWN - NLM STAT- MEDLINE DCOM- 20040903 LR - 20191210 IS - 0266-8254 (Print) IS - 0266-8254 (Linking) VI - 39 IP - 1 DP - 2004 TI - Application of electrospray ionization mass spectrometry in rapid typing of fengycin homologues produced by Bacillus subtilis. PG - 98-102 AB - AIMS: To rapidly type the fengycin homologues produced by Bacillus subtilis strains with electrospray ionization/collision-induced dissociation (ESI/CID) mass spectrometry. METHODS AND RESULTS: Fengycin homologues produced by Bacillus subtilis JA were analysed. When each homologue was subjected to ESI/CID analysis, ions representing characteristic fragmentations were detected. These ions can help to identify the homologues; even homologues of the same nominal mass can be discriminated by their ESI/CID spectra. Based on the CID results, fengycin homologues can be correctly assigned. CONCLUSIONS AND SIGNIFICANCE OF THIS STUDY: ESI/CID leads to rapid detection and structural characterization of fengycin homologues or lipopeptides with similar properties. It will be very useful in studying the regulatory expression of these peptides. FAU - Wang, J AU - Wang J AD - Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Science, Hefei, Anhui, China. wangjun0457@sina.com FAU - Liu, J AU - Liu J FAU - Wang, X AU - Wang X FAU - Yao, J AU - Yao J FAU - Yu, Z AU - Yu Z LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Lett Appl Microbiol JT - Letters in applied microbiology JID - 8510094 RN - 0 (Lipopeptides) RN - 0 (Lipoproteins) RN - 102577-03-7 (fengycin) SB - IM MH - Bacillus subtilis/chemistry/*classification MH - *Bacterial Typing Techniques MH - Lipopeptides MH - *Lipoproteins/chemistry/metabolism MH - Spectrometry, Mass, Electrospray Ionization/*methods EDAT- 2004/06/11 05:00 MHDA- 2004/09/04 05:00 CRDT- 2004/06/11 05:00 PHST- 2004/06/11 05:00 [pubmed] PHST- 2004/09/04 05:00 [medline] PHST- 2004/06/11 05:00 [entrez] AID - LAM1547 [pii] AID - 10.1111/j.1472-765X.2004.01547.x [doi] PST - ppublish SO - Lett Appl Microbiol. 2004;39(1):98-102. doi: 10.1111/j.1472-765X.2004.01547.x.