PMID- 15189347
OWN - NLM
STAT- MEDLINE
DCOM- 20040722
LR  - 20221207
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 89
IP  - 6
DP  - 2004 Jun
TI  - 3,4-Methylenedioxymethamphetamine increases interleukin-1beta levels and 
      activates microglia in rat brain: studies on the relationship with acute 
      hyperthermia and 5-HT depletion.
PG  - 1445-53
AB  - 3,4-Methylenedioxymethamphetamine (MDMA) administration to rats produces acute 
      hyperthermia and 5-HT release. Interleukin-1beta (IL-1beta) is a pro-inflammatory 
      pyrogen produced by activated microglia in the brain. We examined the effect of a 
      neurotoxic dose of MDMA on IL-1beta concentration and glial activation and their 
      relationship with acute hyperthermia and 5-HT depletion. MDMA, given to rats 
      housed at 22 degrees C, increased IL-1beta levels in hypothalamus and cortex from 
      1 to 6 h and 
      [(3)H]-(1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)3-isoquinolinecarboxamide) 
      binding between 3 and 48 h. Increased immunoreactivity to OX-42 was also 
      detected. Rats became hyperthermic immediately after MDMA and up to at least 12 h 
      later. The IL-1 receptor antagonist did not modify MDMA-induced hyperthermia 
      indicating that IL-1beta release is a consequence, not the cause, of the rise in 
      body temperature. When MDMA was given to rats housed at 4 degrees C, hyperthermia 
      was abolished and the IL-1beta increase significantly reduced. The MDMA-induced 
      acute 5-HT depletion was prevented by fluoxetine coadministration but the 
      IL-1beta increase and hyperthermia were unaffected. Therefore, the rise in 
      IL-1beta is not related to the acute 5-HT release but is linked to the 
      hyperthermia. Contrary to IL-1beta levels, microglial activation is not 
      significantly modified when hyperthermia is prevented, suggesting that it might 
      be a process not dependent on the hyperthermic response induced by MDMA.
FAU - Orio, Laura
AU  - Orio L
AD  - Departamento de Farmacologia, Facultad de Medicina, Universidad Complutense, 
      Madrid 28040, Spain.
FAU - O'Shea, Esther
AU  - O'Shea E
FAU - Sanchez, Veronica
AU  - Sanchez V
FAU - Pradillo, Jesus M
AU  - Pradillo JM
FAU - Escobedo, Isabel
AU  - Escobedo I
FAU - Camarero, Jorge
AU  - Camarero J
FAU - Moro, Maria A
AU  - Moro MA
FAU - Green, A Richard
AU  - Green AR
FAU - Colado, M Isabel
AU  - Colado MI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Interleukin 1 Receptor Antagonist Protein)
RN  - 0 (Interleukin-1)
RN  - 0 (Isoquinolines)
RN  - 0 (Serotonin Agents)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 0 (Sialoglycoproteins)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 333DO1RDJY (Serotonin)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - YNF83VN1RL (PK 11195)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Astrocytes/drug effects/metabolism
MH  - Binding, Competitive/drug effects/physiology
MH  - Body Temperature/drug effects
MH  - Brain/*drug effects/metabolism
MH  - Cerebral Cortex/drug effects/metabolism
MH  - Fever/*chemically induced/drug therapy/metabolism
MH  - Fluoxetine/pharmacology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Hypothalamus/drug effects/metabolism
MH  - Interleukin 1 Receptor Antagonist Protein
MH  - Interleukin-1/*metabolism
MH  - Isoquinolines/pharmacokinetics
MH  - Male
MH  - Microglia/*drug effects/metabolism
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Serotonin/deficiency/*metabolism
MH  - Serotonin Agents/pharmacology
MH  - Selective Serotonin Reuptake Inhibitors/pharmacology
MH  - Sialoglycoproteins/pharmacology
MH  - Temperature
EDAT- 2004/06/11 05:00
MHDA- 2004/07/23 05:00
CRDT- 2004/06/11 05:00
PHST- 2004/06/11 05:00 [pubmed]
PHST- 2004/07/23 05:00 [medline]
PHST- 2004/06/11 05:00 [entrez]
AID - JNC2443 [pii]
AID - 10.1111/j.1471-4159.2004.02443.x [doi]
PST - ppublish
SO  - J Neurochem. 2004 Jun;89(6):1445-53. doi: 10.1111/j.1471-4159.2004.02443.x.