PMID- 15191022
OWN - NLM
STAT- MEDLINE
DCOM- 20040708
LR  - 20071114
IS  - 1081-1206 (Print)
IS  - 1081-1206 (Linking)
VI  - 92
IP  - 5
DP  - 2004 May
TI  - Diminished dendritic cell interleukin 10 production in atopic children.
PG  - 538-44
AB  - BACKGROUND: Diminished interleukin 10 (IL-10) and/or IL-12 production may 
      contribute to the pathogenesis of asthma and atopy. Dendritic cells (DCs) produce 
      these cytokines and have been implicated in the pathogenesis of these disorders. 
      OBJECTIVE: To determine whether DC IL-10 and/or IL-12 production is diminished in 
      children aged 6 to 12 years with allergic rhinitis (AR) and with or without 
      asthma. METHODS: Monocyte-derived DCs were isolated from 20 subjects without AR 
      or asthma (group 1), 20 subjects with AR without asthma (group 2), and 20 
      subjects with AR and asthma (group 3). Asthma was defined as a history of 
      physician-diagnosed disease, and AR was defined as a positive history and 
      positive puncture skin test responses (wheal > or = 5 mm) to relevant inhalant 
      allergens. DCs were stimulated with either lipopolysaccharide (LPS) or diluent 
      and cultured for 24 hours. Supernatants were assayed for IL-10 and IL-12 levels 
      by enzyme-linked immunosorbent assay. RESULTS: DC IL-10 production was diminished 
      in groups 2 and 3 compared with group 1. Median LPS-induced IL-10 levels were 
      11.0 pg/mL in group 1, 6.1 pg/mL in group 2, and 1.5 pg/mL in group 3. The 
      frequencies of subjects with detectable IL-10 levels were 85%, 20%, and 20% in 
      groups 1, 2 and 3, respectively. Median LPS-induced IL-12 levels were similar in 
      all groups. CONCLUSIONS: These data support the hypothesis that atopic subjects 
      have an intrinsic inability to up-regulate DC IL-10 production. Future studies in 
      this area could lead to a better understanding of the pathogenesis of atopy.
FAU - Gentile, Deborah A
AU  - Gentile DA
AD  - Division of Allergy, Asthma and Immunology, Department of Pediatrics, Allegheny 
      General Hospital, Pittsburgh, Pennsylvania 15212, USA. dgentile@wpahs.org
FAU - Schreiber, Rachel
AU  - Schreiber R
FAU - Howe-Adams, Judith
AU  - Howe-Adams J
FAU - Trecki, Jordan
AU  - Trecki J
FAU - Patel, Asha
AU  - Patel A
FAU - Angelini, Betty
AU  - Angelini B
FAU - Skoner, David P
AU  - Skoner DP
LA  - eng
GR  - 5M01RR00084/RR/NCRR NIH HHS/United States
GR  - K23RR16072/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Allergy Asthma Immunol
JT  - Annals of allergy, asthma & immunology : official publication of the American 
      College of Allergy, Asthma, & Immunology
JID - 9503580
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Asthma/immunology
MH  - Child
MH  - Dendritic Cells/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology
MH  - Interleukin-10/*biosynthesis/immunology
MH  - Interleukin-12/biosynthesis/immunology
MH  - Male
MH  - Respiratory Function Tests
MH  - Rhinitis, Allergic, Perennial/*immunology
MH  - Skin Tests
EDAT- 2004/06/12 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/06/12 05:00
PHST- 2004/06/12 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/06/12 05:00 [entrez]
AID - S1081-1206(10)61761-9 [pii]
AID - 10.1016/S1081-1206(10)61761-9 [doi]
PST - ppublish
SO  - Ann Allergy Asthma Immunol. 2004 May;92(5):538-44. doi: 
      10.1016/S1081-1206(10)61761-9.