PMID- 15192841
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20190816
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 21
IP  - 3
DP  - 2004 Jun
TI  - [Study on mixed lineage leukemia gene rearrangement in AML-M4/M5 by interphase 
      fluorescence in situ hybridization].
PG  - 288-90
AB  - OBJECTIVE: To explore the value of fluorescence in situ hybridization (FISH) in 
      the detection of mixed lineage leukemia (MLL) rearrangement and to assess the 
      incidence and prognostic significance of MLL gene rearrangement in AML-M4/M5. 
      METHODS: Bone marrow chromosome preparation of 23 cases of acute myeloid 
      leukemia(AML) consisting of 19 cases with M5 and 4 cases with M4 was made using 
      direct method or short-term culture. Karyotypic analysis was carried out by 
      R-banding technique. Dig labeled 11q23 probe which spans the breakpoint cluster 
      region in MLL was used to detect the MLL rearrangement in the above 23 cases. 
      RESULTS: R-banding karyotyping analysis revealed 11q23 translocation in 7 cases, 
      while FISH analysis detected MLL rearrangement in 12 cases including the above 7 
      cases. CONCLUSION: Interphase FISH was more sensitive in detecting the MLL 
      rearrangement in AML-M4/M5, compared with the conventional cytogenetic method. 
      MLL rearrangement is highly related to AML-M4/M5; it is an indication of poor 
      prognosis.
FAU - Pan, Jin-lan
AU  - Pan JL
AD  - The First Affiliated Hospital, Jiangsu Hematology Research Institute, Soochow 
      University, Nanjing, Jiangsu, 215006 PR China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Jiang, Hai-yan
AU  - Jiang HY
FAU - Li, Jian-yong
AU  - Li JY
FAU - Chen, Su-ning
AU  - Chen SN
FAU - Wu, Ya-fang
AU  - Wu YF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Rearrangement
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - *Proto-Oncogenes
MH  - *Transcription Factors
EDAT- 2004/06/12 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/06/12 05:00
PHST- 2004/06/12 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/06/12 05:00 [entrez]
AID - 940621072 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Jun;21(3):288-90.