PMID- 15193420
OWN - NLM
STAT- MEDLINE
DCOM- 20041012
LR  - 20131121
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 125
IP  - 1-2
DP  - 2004 Jun 18
TI  - Molecular cloning and characterization of the promoter region of the human Phox2b 
      gene.
PG  - 29-39
AB  - The closely related homeodomain transcription factors, Phox2a and Phox2b, are 
      restrictively expressed in central and peripheral noradrenergic (NA) neurons in 
      an overlapping but distinct manner, and critically regulate the differentiation 
      and neurotransmitter identity of NA neurons. The structure and function of the 
      human Phox2a (hPhox2a) promoter has recently been reported. Towards the long-term 
      goal of delineating the regulatory cascade of NA neuron differentiation, we 
      isolated a human Phox2b (hPhox2b) genomic clone encompassing approximately 7.8 kb 
      of the 5' upstream promoter region, the entire exon-intron structure and 4.5 kb 
      of the 3' flanking region. Two transcription start sites are identified to reside 
      115 and 110 nucleotides upstream of the start codon, based on both primer 
      extension and 5'-rapid amplification of the cDNA ends analyses. In addition, 
      transient transfection assays indicate that 1.1 kb or longer upstream sequences 
      of the hPhox2b gene may confer cell type-specific gene expression in certain, but 
      not all cell lines. The promoter activity of the hPhox2b gene is modestly 
      transactivated by forced co-expression of Phox2b and the hPhox2b gene promoter 
      contains a high-affinity binding site at -320 to -295 bp. This study provides a 
      frame to further elucidate the molecular mechanisms underlying the regulation of 
      Phox2a and Phox2b gene expression and its relation to NA differentiation.
FAU - Jong Hong, Seok
AU  - Jong Hong S
AD  - Molecular Neurobiology Laboratory, MRC215, McLean Hospital, Harvard Medical 
      School, 115 Mill Street, Belmont, MA 02478, USA.
FAU - Chae, Han
AU  - Chae H
FAU - Kim, Kwang-Soo
AU  - Kim KS
LA  - eng
GR  - DC006501/DC/NIDCD NIH HHS/United States
GR  - MH48866/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Homeodomain Proteins)
RN  - 0 (NBPhox protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Transcription Factors)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Genes, Reporter
MH  - Homeodomain Proteins/*genetics/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Neurons/cytology/metabolism
MH  - Norepinephrine/metabolism
MH  - *Promoter Regions, Genetic
MH  - Rats
MH  - Sequence Alignment
MH  - Transcription Factors/*genetics/metabolism
EDAT- 2004/06/15 05:00
MHDA- 2004/10/13 09:00
CRDT- 2004/06/15 05:00
PHST- 2004/02/08 00:00 [accepted]
PHST- 2004/06/15 05:00 [pubmed]
PHST- 2004/10/13 09:00 [medline]
PHST- 2004/06/15 05:00 [entrez]
AID - S0169328X04001251 [pii]
AID - 10.1016/j.molbrainres.2004.02.023 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2004 Jun 18;125(1-2):29-39. doi: 
      10.1016/j.molbrainres.2004.02.023.