PMID- 15193525
OWN - NLM
STAT- MEDLINE
DCOM- 20040825
LR  - 20131121
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1013
IP  - 2
DP  - 2004 Jul 9
TI  - Protein kinase C inhibition differentially affects 
      3,4-methylenedioxymethamphetamine-induced dopamine release in the striatum and 
      prefrontal cortex of the rat.
PG  - 168-73
AB  - The acute administration of 3,4-methylenedioxymethamphetamine (MDMA) elevates 
      extracellular concentrations of dopamine (DA) and serotonin (5-HT) in the rat 
      striatum and medial prefrontal cortex (mPFC). The release of DA induced by MDMA 
      is thought to involve both transporter and impulse-mediated processes. 
      Furthermore, the impulse-dependent release of DA in the striatum elicited by MDMA 
      appears to involve 5-HT2 receptor activation. Since 5-HT2 receptors are known to 
      utilize protein kinase C (PKC) for intracellular signaling, we examined the 
      effects of modulators of PKC activity on DA release stimulated by MDMA. Reverse 
      dialysis of the PKC inhibitors bisindolylmaleimide I (BIM; 30 microM) or 
      chelerythrine chloride (100 microM) through a microdialysis probe significantly 
      attenuated the MDMA (10 mg/kg, i.p.)-induced increase in the extracellular 
      concentration of DA in the striatum. In contrast, BIM did not significantly alter 
      the increase in the extracellular concentration of DA in the striatum elicited by 
      amphetamine (5 mg/kg, i.p.). Reverse dialysis of a PKC activator, phorbol 
      12,13-dibutyrate (PDBu) (0.5 microM), through the microdialysis probe into the 
      striatum, significantly increased MDMA-induced DA release. In contrast to the 
      inhibitory effects of the PKC inhibitors on MDMA-induced DA release in the 
      striatum, intracortical infusion of BIM enhanced MDMA-induced release of DA in 
      the mPFC. These data suggest that PKC-mediated signaling pathways differentially 
      modulate MDMA-induced DA release from mesocorticolimbic and nigrostriatal 
      neurons.
FAU - Nair, Sunila G
AU  - Nair SG
AD  - College of Pharmacy, University of Cincinnati, 3223 Eden Avenue, Cincinnati, OH 
      45267, USA.
FAU - Gudelsky, Gary A
AU  - Gudelsky GA
LA  - eng
GR  - DA 07427/DA/NIDA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Serotonin Agents)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Corpus Striatum/drug effects/*metabolism
MH  - Dopamine/*metabolism
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Prefrontal Cortex/drug effects/*metabolism
MH  - Protein Kinase C/*antagonists & inhibitors
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin Agents/*pharmacology
MH  - Signal Transduction/physiology
EDAT- 2004/06/15 05:00
MHDA- 2004/08/26 05:00
CRDT- 2004/06/15 05:00
PHST- 2004/04/01 00:00 [accepted]
PHST- 2004/06/15 05:00 [pubmed]
PHST- 2004/08/26 05:00 [medline]
PHST- 2004/06/15 05:00 [entrez]
AID - S0006899304005499 [pii]
AID - 10.1016/j.brainres.2004.04.007 [doi]
PST - ppublish
SO  - Brain Res. 2004 Jul 9;1013(2):168-73. doi: 10.1016/j.brainres.2004.04.007.