PMID- 15193867
OWN - NLM
STAT- MEDLINE
DCOM- 20041223
LR  - 20220318
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 25
IP  - 7
DP  - 2004 Aug
TI  - Placental protein 13 (PP-13): effects on cultured trophoblasts, and its detection 
      in human body fluids in normal and pathological pregnancies.
PG  - 608-22
AB  - Placental tissue protein 13 (PP-13), one of the 56 known placental proteins 
      identified till today, was purified from placentas obtained from women at 
      delivery, and used to evoke antibodies against it. The purified PP-13 was lysed 
      to peptides, which were sequenced, leading to the full-length cDNA sequencing and 
      its expression in Escherichia coli. Sequence analysis in databases showed 
      homology to the galectin family. Of the various antibody preparations developed, 
      a pair of monoclonal antibodies (MAbs) coupled to the recombinant PP-13 (PP-13-R) 
      was used for the immunodetection of PP-13 in pregnant women's serum with the 
      solid-phase ELISA format. With a dynamic range of 25-500 pg/mL with no background 
      in non-pregnant women's serum and men's serum, the ELISA test was suitable for 
      the detection of PP-13 in the 1st, 2nd, and 3rd trimesters. PP-13 levels slowly 
      increase during pregnancy. In the 1st trimester, lower than normal PP-13 levels 
      were found in fetal growth restriction (IUGR), preeclampsia (PE), and 
      particularly in early PE (<34 weeks of gestation). In the 2nd and 3rd trimesters, 
      higher than normal concentrations were found in PE, IUGR and in preterm delivery 
      (PTD). Application of PP-13 to cultured trophoblasts elicited depolarization 
      carried by calcium ions, followed by liberation of linoleic and arachidonic acids 
      from the trophoblast membrane, and a subsequent elevation of prostacyclin and 
      thromboxane. These effects were negligible when PP-13 derived from the placentas 
      of patients with IUGR, PE or PTD was used. The results are discussed in view of 
      the potential utilization of PP-13 for early serum screening to assess the risk 
      to develop placental insufficiency, coupled to a differential analysis of the 
      various pathologies by analyzing cultured trophoblasts.
FAU - Burger, O
AU  - Burger O
AD  - Diagnostic Technology, 49 Ha'Histadrut St., PO Box 25266, Haifa 31250, Israel.
FAU - Pick, E
AU  - Pick E
FAU - Zwickel, J
AU  - Zwickel J
FAU - Klayman, M
AU  - Klayman M
FAU - Meiri, H
AU  - Meiri H
FAU - Slotky, R
AU  - Slotky R
FAU - Mandel, S
AU  - Mandel S
FAU - Rabinovitch, L
AU  - Rabinovitch L
FAU - Paltieli, Y
AU  - Paltieli Y
FAU - Admon, A
AU  - Admon A
FAU - Gonen, R
AU  - Gonen R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Galectins)
RN  - 0 (LGALS13 protein, human)
RN  - 0 (Pregnancy Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amniotic Fluid/chemistry
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Base Sequence
MH  - Body Fluids/*chemistry
MH  - Cells, Cultured
MH  - DNA, Complementary/analysis/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Growth Retardation/metabolism
MH  - Galectins
MH  - Gestational Age
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Obstetric Labor, Premature
MH  - Pre-Eclampsia/metabolism
MH  - Pregnancy
MH  - Pregnancy Complications/*metabolism
MH  - Pregnancy Proteins/*analysis/genetics/*pharmacology
MH  - Radioimmunoassay
MH  - Recombinant Proteins
MH  - Sensitivity and Specificity
MH  - Sequence Homology
MH  - Trophoblasts/*drug effects
EDAT- 2004/06/15 05:00
MHDA- 2004/12/24 09:00
CRDT- 2004/06/15 05:00
PHST- 2003/12/31 00:00 [accepted]
PHST- 2004/06/15 05:00 [pubmed]
PHST- 2004/12/24 09:00 [medline]
PHST- 2004/06/15 05:00 [entrez]
AID - S014340040400013X [pii]
AID - 10.1016/j.placenta.2003.12.009 [doi]
PST - ppublish
SO  - Placenta. 2004 Aug;25(7):608-22. doi: 10.1016/j.placenta.2003.12.009.