PMID- 15193916
OWN - NLM
STAT- MEDLINE
DCOM- 20040729
LR  - 20071114
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 323
IP  - 2
DP  - 2004 Jun 1
TI  - Identification of residues in an orthopoxvirus interleukin-18 binding protein 
      involved in ligand binding and species specificity.
PG  - 197-207
AB  - Interleukin-18 (IL-18) is a critical cytokine in inflammation and adaptive immune 
      responses. The IL-18 binding proteins (IL-18BP) are a family of proteins that 
      bind to, and inhibit the activity of, IL-18. Using point mutagenesis, we analyzed 
      the ectromelia virus IL-18BP to identify residues involved in binding. Because 
      p13 can bind both human and murine IL-18, and because it is highly homologous to 
      the variola virus IL-18BP, we set out to identify residues that may be involved 
      in species specificity. Several of the mutations resulted in complete abrogation 
      of binding affinity. Three (F49A, E77A, and E69A) significantly affected binding 
      with both species of IL-18, but not to the same extent. Mutant H70A showed 
      reduced affinity for human IL-18 while binding to murine IL-18 was not affected. 
      This study demonstrated that interaction of IL-18 with p13 was similar to other 
      IL-18BPs, however, novel species-specific interactions were identified.
FAU - Esteban, David J
AU  - Esteban DJ
AD  - Department of Molecular Microbiology and Immunology, St. Louis University Health 
      Sciences Center, St. Louis, MO 63104, USA.
FAU - Buller, R Mark L
AU  - Buller RM
LA  - eng
GR  - U01 48653-04/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Ligands)
RN  - 0 (interleukin-18 binding protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Ectromelia virus/*pathogenicity
MH  - Glycoproteins/*chemistry/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Interleukin-18/*metabolism
MH  - Ligands
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Point Mutation
MH  - Protein Binding
MH  - Species Specificity
MH  - Surface Plasmon Resonance
EDAT- 2004/06/15 05:00
MHDA- 2004/07/30 05:00
CRDT- 2004/06/15 05:00
PHST- 2003/11/03 00:00 [received]
PHST- 2003/12/16 00:00 [revised]
PHST- 2004/02/27 00:00 [accepted]
PHST- 2004/06/15 05:00 [pubmed]
PHST- 2004/07/30 05:00 [medline]
PHST- 2004/06/15 05:00 [entrez]
AID - S0042682204001631 [pii]
AID - 10.1016/j.virol.2004.02.027 [doi]
PST - ppublish
SO  - Virology. 2004 Jun 1;323(2):197-207. doi: 10.1016/j.virol.2004.02.027.