PMID- 15195102
OWN - NLM
STAT- MEDLINE
DCOM- 20050124
LR  - 20180816
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 22
IP  - 7
DP  - 2004 Jul
TI  - Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an 
      engineered secretory laccase.
PG  - 893-7
AB  - Plant roots release a range of enzymes capable of degrading chemical compounds in 
      their immediate vicinity. We present a system of phytoremediation ex planta based 
      on the overexpression of one such enzyme, a secretory laccase. Laccases catalyze 
      the oxidation of a broad range of phenolic compounds, including polychlorinated 
      phenols such as 2,4,6-trichlorophenol (TCP), that are among the most hazardous 
      and recalcitrant pollutants in the environment. We isolated a secretory laccase 
      cDNA of LAC1, which is specifically expressed in the roots of Gossypium arboreum 
      (cotton). Transgenic Arabidopsis thaliana plants overexpressing LAC1 exhibited 
      enhanced resistance to several phenolic allelochemicals and TCP. The secretory 
      laccase activity in these plants was responsible for the conversion of sinapic 
      acid into a mono-lactone type dimer and for the transformation of TCP.
FAU - Wang, Guo-Dong
AU  - Wang GD
AD  - National Key Laboratory of Plant Molecular Genetics, Institute of Plant 
      Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese 
      Academy of Sciences, 300 Fenglin Road, Shanghai 200032, P.R. China.
FAU - Li, Qian-Jin
AU  - Li QJ
FAU - Luo, Bin
AU  - Luo B
FAU - Chen, Xiao-Ya
AU  - Chen XY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040613
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (Chlorophenols)
RN  - 0 (Coumaric Acids)
RN  - 0 (Hydroxybenzoates)
RN  - 0 (Pheromones)
RN  - 632XD903SP (Gallic Acid)
RN  - 68A28V6010 (sinapinic acid)
RN  - E390O181H5 (syringic acid)
RN  - EC 1.10.3.2 (Laccase)
RN  - GM8Q3JM2Y8 (Vanillic Acid)
RN  - I3P9R8317T (phenolic acid)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Arabidopsis/*genetics/metabolism
MH  - Biotransformation
MH  - Chlorophenols/*metabolism/pharmacology
MH  - Cloning, Molecular
MH  - Coumaric Acids/analysis
MH  - Drug Resistance
MH  - Gallic Acid/*analogs & derivatives/analysis
MH  - Gossypium/*enzymology/genetics
MH  - Hydroxybenzoates/*metabolism/pharmacology
MH  - Laccase/*genetics/metabolism
MH  - Pheromones/*metabolism/pharmacology
MH  - Plant Roots/enzymology
MH  - Plants, Genetically Modified/genetics/metabolism
MH  - Protein Engineering
MH  - Vanillic Acid/analysis
EDAT- 2004/06/15 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/06/15 05:00
PHST- 2004/02/10 00:00 [received]
PHST- 2004/03/03 00:00 [accepted]
PHST- 2004/06/15 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/06/15 05:00 [entrez]
AID - nbt982 [pii]
AID - 10.1038/nbt982 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2004 Jul;22(7):893-7. doi: 10.1038/nbt982. Epub 2004 Jun 13.